Sequences from confirmation amplicon


Confirmation sequences for line 352E04 and hit At5g15180 are shown.
Line ID 352E04
Locus At5g15180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4931117
Primers used for PCR BV68 - 8474, show primer details
   
Sequence Name 38-K106149-0022-352-E04C-BV68
GenBank Accession LN502986 [GenBank]
Sequencing primer gene specific primer, BV68: 5'-AGTTGCTAGTCCAAAATAGCCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K106149-0022-352-E04C-BV68
GTTCAAGAACCTGCCCTTTAATTATTTGATGCATCGTTCAAGTATAATTTAAAGAATTTT
CTGATTCCATAACATACCTGAAAGCACAACGAGGTCTTTCTTGTCGAGGCCTTTGGACTG
GAATTGAGTGATAAGGCTACTGATATTATTAAATGGTGATGGTAAGTTTAATAAGGCCTC
AGTGATGTTTGTAACCAGACCGTCTCTTCGTCCCGTTTCAACCTCCCACGACGGTCCATT
GAGCTGAACATATATTGATTGGTTATCATAAAATACATGATGTTAAAAGTTCAACACAAC
ATATAATACATAATTTTTTTTATGGATATATTTTCTTTAATATATTAGTCAGAAAATCTC
AGATAGAAAGCTGAACTAGTCTCTTGAAAATTTAAGTCAAGTGCATTTTGTAGCATCAAA
AAAATAGAAGAATACGCCCAACCCTATTTATGATGTCTATAATCGCCATGTGAACTTTGT
AGTAATAAATACAAGGAGCGTGTAATAAAGAAAAAGAAAGAGTATGATGGTCAATATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 54-K106150-0022-352-E04C-8409
GenBank Accession LN508484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106150-0022-352-E04C-8409
ATTGACATCATACTCTTTCTTTTTCTTTATTACACGCTCCTTGTATTTATTACTACAAAG
TTCACATGGCGATTATAGACATCATAAATAGGGTTGGGCGTATTCTTCTATTTTTTTGAT
GCTACAAAATGCACTTGACTTAAATTTTCAAGAGACTAGTTCAGCTTTCTATCTGAGATT
TTCTGACTAATATATTAAAGAAAATATATCCATAAAAAAAATTATGTATTATATGTTGTG
TTGAACTTTTAACATCATGTATTTTATGATAACCAATCAATATATGTTCAGCTCAATGGA
CCGTCGTGGGAGGTTGAAACGGGACGAAGAGACGGTCTGGTTACAAACATCACTGAGGCC
TTATTAAACTTACCATCACCATTTAATAATATCAGTAGCCTTATCACTCAATTCCAGTCC
AAAGGCCTCGACAAGAAAGACCTCGTTGTGCTTTCAGGTATGTTATGGAATCAGAAAATT
CTTTAAATTATACTTGAACGATGCATCAAATAATTAAAGGGCAGTGTTCTTTGAACAAAT
GTTTTTCTCTTTTTTCCAAGCATTGTTTGGCTATTTTGGACTAGCAA

Last Updated on 06.12.2018 16:11