Sequences from confirmation amplicon


Confirmation sequences for line 353A11 and hit At3g21240 are shown.
Line ID 353A11
Locus At3g21240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7455430
Primers used for PCR HF15 - 8474, show primer details
   
Sequence Name 38-K032416-022-353-A11C-8409
GenBank Accession LN502880 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K032416-022-353-A11C-8409
TCAATTGTGTTTGAAGTTTTAAAAAATTGAAGACTAAAACGGCTCTGTTTCTAAACTTAA
CGTTTTGGATGTTGGTTACTACGAGCAGGGCTATGGGATGACAGAAGCAGGTCCGGTGCT
AGCAATGTCGTTAGGGTTTGCTAAAGAGCCGTTTCCAGTGAAGTCAGGAGCATGTGGTAC
GGTGGTGAGGAACGCCGAGATGAAGATACTTGATCCAGACACAGGAGATTCTTTGCCTAG
GAACAAACCCGGCGAAATATGCATCCGTGGCAACCAAATCATGAAAGGCTATCTCAATGA
CCCCTTGGCCACGGCATCGACGATCGATAAAGATGGTTGGCTTCACACTGGAGACGTCGG
ATTTATCGATGATGACGACGAGCTTTTCATTGTGGATAGATTGAAAGAACTCATCAAGTA
CAAAGGATTTCAAGTGGCTCCAGCTGAGCTAGAGTCTCTCCTCATAGGTCATCCAGAAA
   
Sequence Name 38-K032416-022-353-A11C-HF15
GenBank Accession LN502881 [GenBank]
Sequencing primer gene specific primer, HF15: 5'-CAACATCATTGATTTCTGGATGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K032416-022-353-A11C-HF15
CTTGAATCCTTTGTACTTGATGAGTTCTTTCAATCTATCCACAATGAAAAGCTCGTCGTC
ATCATCGATAAATCCGACGTCTCCAGTGTGAAGCCAACCATCTTTATCGATCGTCGATGC
CGTGGCCAAGGGGTCATTGAGATAGCCTTTCATGATTTGGTTGCCACGGATGCATATTTC
GCCGGGTTTGTTCCTAGGCAAAGAATCTCCTGTGTCTGGATCAAGTATCTTCATCTCGGC
GTTCCTCACCACCGTACCACATGCTCCTGACTTCACTGGAAACGGCTCTTTAGCAAACCC
TAACGACATTGCTAGCACCGGACCTGCTTCTGTCATCCCATAGCCCTGCTCGTAGTAACC
AACATCCAAAACGTTAAGTTTAGAAACAGAGCCGTTTTAGTCTTCAATTTTTTAAAACTT
TCAAACACAATTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACT

Last Updated on 06.12.2018 16:11