Sequences from confirmation amplicon


Confirmation sequences for line 353E02 and hit At4g21270 are shown.
Line ID 353E02
Locus At4g21270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11331377
Primers used for PCR PC13 - 8474, show primer details
   
Sequence Name 04-K037024-022-353-E02C-PC13
GenBank Accession LN487828 [GenBank]
Sequencing primer gene specific primer, PC13: 5'-ATGACCTTCAGATGCTCTTGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K037024-022-353-E02C-PC13
ATTTATACTGTGGATACAGATGGCAGATGCATCAGTATCTCTTACTAGGACAGAGTTTGA
GGAGCAGAAGCATCTACTGTGCGAACTACAAGACCGGCTTGCAGACATGGAGCATCAACT
GTGTGAGGGAGAGTTATTGCGGAAGAAGCTTCATAACACTATTCTTGTAAGAAACTATTT
TTTTTTCCCTTATATATGTGGCTGTCTTGATCTGAGCTAAGAGAAGCTGTAAATTCAGGA
ATTAAAAGGAAATATACGTGTCTTCTGCCGAGTGCGTCCCTTGTTACCAGACGATGGAGG
ACGACATGAAGCAACTGTAATAGCCTATCCTACTTCAACAGAAGCCCAAGGAAGAGGTGT
TGATTTGGTTCAGAGTGGTATGACTATTTCCTGACTTGAATTACCAATTGTCAAAGCAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACT
CCCAA
   
Sequence Name 03-K037024-022-353-E02C-8409
GenBank Accession LN487393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K037024-022-353-E02C-8409
CATTGCTTTGAAATTGGTAATTCAAGTCAGGAAATAGTCATACCACTCTGAACCAAATCA
ACACCTCTTCCTTGGGCTTCTGTTGAAGTAGGATAGGCTATTACAGTTGCTTCATGTCGT
CCTCCATCGTCTGGTAACAAGGGACGCACTCGGCAGAAGACACGTATATTTCCTTTTAAT
TCCTGAATTTACAGCTTCTCTTAGCTCAGATCAAGACAGCCACATATATAAGGGAAAAAA
AAATAGTTTCTTACAAGAATAGTGTTATGAAGCTTCTTCCGCAATAACTCTCCCTCACAC
AGTTGATGCTCCATGTCTGCAAGCCGGTCTTGTAGTTCGCACAGTAGATGCTTCTGCTCC
TCAAACTCTGTCCTAGTAAGAGATACTGATGCATCTGCCATCTGTATTCACAGTTATAAA
TATCTATCAAATTAGCTATTTGAAATCAAGAGCATCTGAAGGTC

Last Updated on 06.12.2018 16:11