Sequences from confirmation amplicon


Confirmation sequences for line 354E09 and hit At4g08980 are shown.
Line ID 354E09
Locus At4g08980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5760032
Primers used for PCR FS02 - 8474, show primer details
   
Sequence Name 07-K020608-022-354-E09C-8409
GenBank Accession LN489063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K020608-022-354-E09C-8409
TGCTCAAACTCATCCTCATAGAACTCTCCCATTACACCATCTTCAAAAAACTCCCAAGCC
AAGTAATCAGACCCATCTGAGAAGTAATCTGAGCAGCAATCCTCCCAATCATTTATCATA
TCATAGAATCCGATCACGCGTGGACCTAACACTTTCATATCCGGAAACTTCTCTTTGAAA
AACTTGTTATCAAGTTGCACGTCCCAACAGCCTCTAAGTTCCAAGAACTCAAGAAAAACA
CAGCACGATAAGATCTTGAGTACTCCTTCTGTGCTGACTCGGTGGTAAGCTATCTCTAAC
CGCTTCAGCTTCGGCATTGTATTGGCGATAGCATAAGCTTCATCGTCGTGAGAGACAACA
CTTGCTACATCCAATGGATGCATGTTTCTGCAAAACTCTCTCAGTGATTTACAGTGTTTT
CCTATGGCTTGTATCGCCTCTGGGCCTATTTTGCAGCAGTAGCTCAAATCCAAGAAAGTG
AGAGACGAGAGCTTTTCAGCTACGTTTACCACACCCGAATTAGTCAGACCACTTCTTGGC
ACCTTCAAGGTCTTAAGTGAACCAGCACTTACCAAACAAAACAAGAAAACATGTAAAATA
TTAGATACAAAAGCAATGAACTAAGAAATCATGTTGCTTCAATCACATCCCCTAATACAT
TCTATGCCTATAACCACTTAAAAGCTTATTCTAAATATGATCATAATACATTAAACTATA
GCAACTTACTGTTGTGCAATGAAGGAGAAGATAGAGTCAT
   
Sequence Name 08-K020608-022-354-E09C-FS02
GenBank Accession LN489531 [GenBank]
Sequencing primer gene specific primer, FS02: 5'-TCCAGATGCTCTTGGTTTAATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K020608-022-354-E09C-FS02
CACTTACCTCTTCAAGAAGTACTAACAGTGGTGCCTAGAGTTTGCAAAGCATGGAACAGA
GCTGTTACTGGACCTTATTGTTGGCAAGAGATTGACATTGAGCTGTGGAGTAACCGTTTT
CACCAGTCTGATCATCTTGACCGGATGCTTGAGATGTTGATCCCTAGAAGCGCTGGCTCT
TTGCGAAAACTCTCCGTTACTGGCCTTCGAAATGACTCTATCTTCTCCTTCATTGCACAA
CAGTAAGTTGCTATAGTTTAATGTATTATGATCATATTTAGAATAAGCTTTTAAGTGGTT
ATAGGCATAGAATGTATTAGGGGATGTGATTGAAGCAACATGATTTCTTAGTTCATTGCT
TTTGTATCTAATATTTTACATGTTTTCTTGTTTTGTTTGGTAAGTGCTGGTTCACTTAAG
ACCTTGAAGGTGCCAAGAAGTGGTCTGACTAATTCGGGTGTGGTAAACGTAGCTGAAAAG
CTCTCGTCTCTCACTTTCTTGGATTTGAGCTACTGCTGCAAAATAGGCCCAGAGGCGATA
CAAGCCATAGGAAAACACTGTAAATCACTGAGAGAGTTTTGCAGAAACATGCATCCATTG
GATGTAGCAAGTGTTGTCTCTCACGACGATGAAGCTTATGCTATCGCCAATACAATGCCG
AAGCTGAAGCGGTTAGAGATAGCTTACCACCGAGTCAGCACAGAAGGAGTACTCAAGATC
TTAT

Last Updated on 06.12.2018 16:11