Sequences from confirmation amplicon


Confirmation sequences for line 354F12 and hit At5g49040 are shown.
Line ID 354F12
Locus At5g49040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19883112
Primers used for PCR 28rq - 8474, show primer details
   
Sequence Name 61-K116102-0022-354-F12C-28rq
GenBank Accession LN510600 [GenBank]
Sequencing primer gene specific primer, 28rq: 5'-CCAGTCTTAAAAGCCAAGTTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K116102-0022-354-F12C-28rq
TTTGGGCGCTCACGTACATCCCTTGGGCTTGACCCACCACAGTCGAGTTAATGGGCACAT
CTAATGTTAAAGCGTTGTCTATCATGGTGATTGATCCGAAATATGAGGAACTTGAGTATT
TTGCAACCGGCCCTTGGATCCTGATGGAACTTGGGTTTCGACCAGTTACAATGTCGTGCC
AATAGACACGGAGATCTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 43-K116101-0022-354-F12C-8409
GenBank Accession LN504958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116101-0022-354-F12C-8409
AATTGAATAGATCTCCGTGTCTATTGGCACGACATTGTAACTGGTCGAAACCCAAGTTCC
ATCAGGATCCAAGGGCCGGTTGCAAAATACTCAAGTTCCTCATATTTCGGATCAATCACC
ATGATAGACAACGCTTTAACATTAGATGTGCCCATTAACTCGACTGTGGTGGGTCAAGCC
CAAGGGATGTACGTTGGAGCAGCCCAAAAGGAGATAGGGTTGTTGATGGCGATGAACTTG
GCTTNTAAGACTGGA

Last Updated on 06.12.2018 16:11