Sequences from confirmation amplicon


Confirmation sequences for line 355B11 and hit At5g54400 are shown.
Line ID 355B11
Locus At5g54400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22090650
Primers used for PCR JZ35 - 8474, show primer details
   
Sequence Name 08-K103223-0022-355-B11C-8409
GenBank Accession LN489691 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K103223-0022-355-B11C-8409
TTCGTAAAAAAATTGAAATCTTCGCATCAATGGGGAAGACAACGAATGGAACAAGAGACT
GGGCTCAGATCTACGCAATCTACGGGATCGAGCAAAAACACACACTCATCTTCCTGATAC
TCAACGCGATTGCTTTCTCTATACTCTCCACCGTCTTTGTCATCTACTTTAACCCGATCT
GCGTCTTCTTTCAATCGTTTCTCTTCTCTAACGCCGCGGCAGCTAGATTCTCGGCTGGAT
TCTTTGGAGCTGTTACGGCGCTTTCCGCCGTGTGTTTGTTTTTCGCGGCGGCGAATTTTT
TTTACTCTGCTGTGCCGCTTAGGTATGAGATGGCGCAAAGGATGGTTGGATCTGTTGGAG
ATTGGTCGAGTGTGAAGACCGCTCTTGACTTAGGTTGTGGTCGTGGGATTTTGCTTAATG
CCGTTGCGACGCANCTCAAGAAAACTGGTAGCTCGGGTCGGGTCGTTGGGTTGGATCGGT
CCATGACTACNACTCTCTCTACTCTACGTACAGCTCACATCGAAGGTTCGTTAGTTCTTT
TTTTTTACGTTGACTTTTTTGACTTATTTCAAAGTCGTTTCTCGTTGACCAACATATTAT
TTTGTAGATTTCCAACTA
   
Sequence Name 56-K103230-0022-355-B11C-JZ35
GenBank Accession LN509070 [GenBank]
Sequencing primer gene specific primer, JZ35: 5'-ACCCGTTAATCACGCAATATAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103230-0022-355-B11C-JZ35
TTGGTCACGAGAACGACTTTGAATAAGTCAAAAAGTCAACGTAAAAAAAAAGAACTAACG
AACCTTCGATGTGAGCTGTACGTAGAGTAGAGAGAGTAGTAGTCATGGACCGATCCAACC
CAACGACCCGACCCGAGCTACCAGTTTTCTTGAGCTGCGTCGCAACGGCATTAAGCAAAA
TCCCACGACCACAACCTAAGTCAAGAGCGGTCTTCACACTCGACCAATCTCCAACAGATC
CAACCATCCTCTGCGCCATCTCATACCTAAGCGGCACAGCAGAGTAAAAAAAATTCGCCG
CCGCGAAAAACAAACACACGGCGGAAAGCGCCGTAACAGCTCCAAAGAATCCAGCCGAGA
ATCTNGCTGCCGCGGCGTTNGAGAAGAGAAACGATTGAAAGAAGACGCAGATCGGGTTAA
AGTAGATGACAAAGACGGTGGAGAGTATAGAGAAAGCAATCGCGTTGAGTATCAGGAAGA
TGAGTGTGTGTTTTTGCTCGATCCCGTAGATTGCGTAGATCTGAGCCCAGTCTCTTGTTC
CATTCGTTGTCTTCCCCATTGATGCGAAGATTTCAATGTTTTTTTACGAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCCGCAGCGTTNTTATAAAAT
GAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11