Sequences from confirmation amplicon


Confirmation sequences for line 355F11 and hit At2g04300 are shown.
Line ID 355F11
Locus At2g04300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1496509
Primers used for PCR EQ35 - 8474, show primer details
   
Sequence Name 08-K103004-0022-355-F11C-8409
GenBank Accession LN489688 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K103004-0022-355-F11C-8409
ATTTACATTGAATATATCCTGTGAGATCATCACAAACCAACATGTGATCAACCAAAGTCG
TGAAAAACCACATATAGCTGAATGGGTTGGGGTGATGCTTACAAAAGGAGACATCAAAAG
CATTATAGATCCAAAATTTAGTGGAGATTATGATGCTGGTTCTGTCTGGAGAGCAGTTGA
ACTAGCAATGTCGTGTGTAAATCCTTCTTCAACTGGAAGACCAACCATGTCTCAAGTTGT
AATCGAATTAAATGAATGTTTGGCATCAGAAAACTCAAGGAGAGGAATGAGTCAAAACAT
GGAGTCAAAGGGATCTATCCNATATACAGAAGTCAGCACGA
   
Sequence Name 56-K103005-0022-355-F11C-EQ35
GenBank Accession LN509068 [GenBank]
Sequencing primer gene specific primer, EQ35: 5'-TTCGTGCTGACTTCTGTATATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103005-0022-355-F11C-EQ35
TCCTCTCCTTGAGTTTTCTGATGCCAACATTCATTTAATTCGATTACAACTTGAGACATG
GTTGGTCTTCCAGTTGAAGAAGGATTTACACACGACATTGCTAGTTCAACTGCTCTCCAG
ACAGAACCAGCATCATAATCTCCACTAAATTTTGGATCTATAATGCTTTTGATGTCTCCT
TTTGTAAGCATCACCCCAACCCATTCAGCTATATNTGGTTTTTCACGACTTTGGTTGATC
ACATGTTGGTTTGTGATGATCTCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACNTTCACTCC
Primers used for PCR 37wy - 3144, show primer details
   
Sequence Name 30-K118781-0022-355-F11C-35St
GenBank Accession LN499929 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118781-0022-355-F11C-35St
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCGAT
AATATCTGCAAAATAGTTTCTTTATACCATTAGAAAAGCGAAAAAATGCAAAACAATATT
GTATGAATTAAGCGATGAAAGACGAGAGCCAATAATATTGATCTCCAAACTAATTAATAA
ATTACCAAACTGGTAAGCTACAAACATATAACACTTACTCTGGATCAAGGTACCCAGGAG
TTCCAGCAACAACTGTGTCCACACGGGTTTCTCCTTCAAGAGGAAAAGATCTCGAAAGAC
CGAAATCAGCAAGCTTGGCCTGAAAATGTTCATCCAACAATATATTTGTGGTTTTGACAT
CTCTATGTACCATTGGTGGTTTGCATCCATTATGCAAGTATTCCAGCCCTATATAGTAAA
TTTTATTTTCATCAAGGCTTAAGCAAAGAACAAGAGGAAATCAAGAAAATATGTATGTCA
AAACTCATTAAACCTTGCGCGGACTCGGCCACTATCTTTAGTCTAGTTTTCCAGTCCAAA
ATAGATACACCTTGGTTTCCTACAGTTAATTCGAGATACAAATATGAAAAAAAAAAAAAC
CAGAAACCAACCGGGTTTTTGTTAAAACCTCTTAAATTAACCTTCCCAAAAAAATTTCCT
TAAAAATCCCCTTAACCCTTGACCTCGAAAATAAAAAATAATTT
   
Sequence Name 70-K118782-0022-355-F11C-37wy
GenBank Accession LN512472 [GenBank]
Sequencing primer gene specific primer, 37wy: 5'-TATCATGGAACTGTGAATGATGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118782-0022-355-F11C-37wy
CTCATATCTCAGGGTATAAGAATTCAAAGCAGAGGTACTTTATACAACAATTTTAGATTA
CAAATTTGTAGAAAAATCAATGTTCAATAAGATGTGATTTGTTAAATCTTGAGTTGAAAT
TCTTTCTACCCTTTCAGGTAGAACTCCTTCTCAGAGTTCACCATAAAAATTTGGTTGGCC
TCGTTGGATATTGTGATGAAGGAGAAAATTTATCTCTCATCTACGAGTACATGGCTAAAG
GAGATCTTAAAGAACATATGTTAGGTAAGTATAATTAAAGAGTTTCTACTAATATCACGT
TTGTTTCTTGTTTTTTTTTTTTTCCAATTTGGTTCCCCAAATAACCGGAGGAAACCCAGG
GGGTTTTTTTTTGGGATGGAAAACTTAAACAAAAAATGGGGCCCAATCCCCCCAAGGGTT
AAAGGATTTTGAACAAACATTTTTCCTGAATTCCCCCTGGTCCTTGGCTAAACCTTGGAG
AAAAAAAAATTTTCCTATATGGGGTGGAAATCTTGGCAAAGGGGAGCCAACCCCCCAGGG
GTCCTTAAAATTTTAAAACCCCCAAATTTTTGGTGGAAAAAACTTTTTCGGGCAAACTTG
GTAAATTTGGGTTTTCCAAAATTTTTCCCTTTGAAAGAAAAACCCCGTGGGAACACATTT
TTGGTGGAACCCCCGGGGACCTTGAACCAAAAGAAGGTTTAATTTTTTTGAACTTACCCA
TTTGGGAAATTAATAAATAATTTGGAAAA

Last Updated on 06.12.2018 16:11