Sequences from confirmation amplicon


Confirmation sequences for line 356B07 and hit At5g26010 are shown.
Line ID 356B07
Locus At5g26010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9086946
Primers used for PCR AB66 - 8474, show primer details
   
Sequence Name 25-K105138-0022-356-B07C-AB66
GenBank Accession LN497437 [GenBank]
Sequencing primer gene specific primer, AB66: 5'-AAATGTACCTGAGTTATGGCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105138-0022-356-B07C-AB66
GCTCTCTATCAATGAGTCTAAAGGCAGTAAAACAAGCCTTTTCCCATTTTGAAGCTTCTT
CTTCACAAACATTAGATTCTTGGTTTAGCTCTTCCTTTAGTGCTAACAAAACCGATGGCA
ATCTATTCCTCACCATTTTGCTCACCATGTGCCCATTTTTGCCGTGTCCATCAAACACTC
CACATAGCTCTGTATCTCTTGTTCCATATCCCTACGGGATTAAAATTAAACAATTTCTAC
TATTTAAATCTAATCAGTTCACTTAATTCTACTCAATAATAAGATAGATGAAACTTTAAA
TACATTTTTCAATCTGCGTGAGAAATGTCATGTGAAATGAATGTAATTTTAACTTTATAT
ATAAACTTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCC
   
Sequence Name 09-K105139-0022-356-B07C-8409
GenBank Accession LN490170 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K105139-0022-356-B07C-8409
CCATTTACATTGAATATAAGTTTATATATAAAGTTAAAATTACATTCATTTCACATGACA
TTTCTCACGCAGATTGAAAAATGTATTTAAAGTTTCATCTATCTTATTATTGAGTAGAAT
TAAGTGAACTGATTAGATTTAAATAGTAGAAATTGTTTAATTTTAATCCCGTAGGGATAT
GGAACAAGAGATACAGAGCTATGTGGAGTGTTTGATGGACACGGCAAAAATGGGCACATG
GTGAGCAAAATGGTGAGGAATAGATTGCCATCGGTTTTGTTAGCACTAAAGGAAGAGCTA
AACCAAGAATCTAATGTTTGTGAAGAAGAAGCTTCAAAATGGGAAAAGGCTTGTTTTACT
GCCTTTAGACTCATTGATAGAGAGCTTAATCTTCAAGTCTTTAATTGCTCTTTCAGTGGC
TCCACTGGTGTGGTTGCCAT

Last Updated on 06.12.2018 16:11