Sequences from confirmation amplicon


Confirmation sequences for line 356B08 and hit At5g63490 are shown.
Line ID 356B08
Locus At5g63490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25419069
Primers used for PCR CF86 - 8474, show primer details
   
Sequence Name 53-K106290-0022-356-B08C-8409
GenBank Accession LN508164 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K106290-0022-356-B08C-8409
GCATGCAAATCATTGGCTGGAAGGTAAAAAGAACGATTTCCTCCTGCTTTAAACTTTTGG
TTGATAAGCAACAAGAACCAAAGTCGAAGATGAAACCTTCATGAGATTGCCCATAAACCC
CTTTTGCTTTCTGAATTGATTTGATCATTATAAATTGCAGAGTCTGAGATTACATTTGGA
TGATTCAAGAGAAGGCAAAGGAAGAAGAAGGAGAAGAGCATCAGGATCAGCAGAATCAAT
GGAGTATGTAGAGACAGACGCTTGGGCTGCTGCTTATAGTGGAGTTGCGGCTGGTGCAGC
ATTAGTAGCTGGTCTTGGTTTTATGGCTTTTCTCAGAAAATTCGGTCATTGATTCCTCAG
AAACACTACAAGAAGTGTTTCTTTAACAGATCAATACAAACAAAAAAGAAAAACAGACAC
AGGGTTTGAAGGTTTTGTCTGAAATATTTTTATATACAAAAGTCATTTTTGATTATTTTT
TTGGCTTCATGATATCAAAATAACAAATATCTAAAACTAATACATGGAGTAATTCAAGAA
CAAGGCTTAGAATCTTTGTCTAAACTGATCTCACTATTGTCAAAAAAATAAAACTCAAAC
AGA
   
Sequence Name 53-K106291-0022-356-B08C-CF86
GenBank Accession LN508165 [GenBank]
Sequencing primer gene specific primer, CF86: 5'-AGCCCTTGTTCTGTTTGAGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K106291-0022-356-B08C-CF86
TTAGACAAGATTCTAGCCTTGTTCTTGAATACTCCATGTATTAGTTTTAGATATTTGTTA
TTTTGATATCATGAAGCCAAAAAAATAATCAAAAATGACTTTTGTATATAAAAATATTTC
AGACAAAACCTTCAAACCCTGTGTCTGTTTTTCTTTTTTGTTTGTATTGATCTGTTAAAG
AAACACTTCTTGTAGTGTTTCTGAGGAATCAATGACCGAATTTTCTGAGAAAAGCCATAA
AACCAAGACCAGCTACTAATGCTGCACCAGCCGCAACTCCACTATAAGCAGCAGCCCAAG
CGTCTGTCTCTACATACTCCATTGATTCTGCTGATCCTGATGCTCTTCTCCTTCTTCTTC
CTTTGCCTTCTCTTGAATCATCCAAATGTAATCTCAGACTCTGCAATTTATAATGATCAA
ATCAATTCAGAAAGCAAAAGGGGTTTATGGGCAATCTCATGAAGGTTTCATCTTCGACTT
TGGTTCTTGTTGCTTATCAACCAAAAGTTTAAAGCAGGAGGAAATCGTTCTTTTTACCTT
CCAGCCAATTGATTTTGCATGCTCTAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
C

Last Updated on 06.12.2018 16:11