Sequences from confirmation amplicon


Confirmation sequences for line 356D03 and hit At3g20110 are shown.
Line ID 356D03
Locus At3g20110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7022399
Primers used for PCR MK43 - 8474, show primer details
   
Sequence Name 03-K101324-0022-356-D03C-8409
GenBank Accession LN487415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101324-0022-356-D03C-8409
GAGCCTTTACAATTGAAGCTTATGATATATTCCCATGCATTGATATGGTCGTGGTAATCA
AAATTTCTTCTCATTAAGTGTGGATTCATTAAGAATGGGGAAAAACAAAGTCTGACTAAG
AAACGTTTGAAACTTGCAGGAGCTTTTACTTGGAGGCACTGATACCTCGGCGCAAACAAT
ACAGTGGACAATGGCCGAGCTCATCAACAACCGTAACGTTCTTAAGAGATTGAGAGAAGA
AATTGATTCGGTTGTAGGAGAAACAAGGTTGATTCAAGAAAAGGATCTACCAAAGCTACC
TTATTTACAATCTGTAGTCAAAGAAGGGCTAAGATTGCACCCGCCATTGCCTCTCATGGT
AAGGACGTTCCAAAGAAGTTGTGAGATGAAAGGGTTCTACATAGC
   
Sequence Name 03-K101341-0022-356-D03C-MK43
GenBank Accession LN487416 [GenBank]
Sequencing primer gene specific primer, MK43: 5'-CGCTATGTAGAACCCTTTCATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101341-0022-356-D03C-MK43
CATGGCGGGTGCATCTTAGCCCTTCTTTGACTACGATTGTAAATAAGGTAGCTTTGGTAG
ATCCTTTTCTTGAATCAACCTTGTTTCTCCTACAACCGAATCAATTTCTTCTCTCAATCT
CTTAAGAACGTTACGGTTGTTGATGAGCTCGGCCATTGTCCACTGTATTGTTTGCGCCGA
GGTATCAGTGCCTCCAAGTAAAAGCTCCTGCAAGTTTCAAACGTTTCTTAGTCAGACTTT
GTTTTTCCCCATTCTTAATGAATCCACACTTAATGAGAAGAAATTTTGATTACCACGACC
ATATCAATGCATGGGAATATATTCATAAGCTTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCANAAATGTAGA

Last Updated on 06.12.2018 16:11