Sequences from confirmation amplicon


Confirmation sequences for line 356E04 and hit At3g56080 are shown.
Line ID 356E04
Locus At3g56080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20812515
Primers used for PCR 23sj - 8474, show primer details
   
Sequence Name 61-K116549-0022-356-E04C-23sj
GenBank Accession LN510605 [GenBank]
Sequencing primer gene specific primer, 23sj: 5'-TTATCAGGAAAAGGAAGCTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K116549-0022-356-E04C-23sj
GAACTGAATCTGAGCTTCGTGTTCGTCTTTAGGAGCAAATGACATTGTGATAACGTTCTT
ATCAAGTAAAGTCCCACCAAAGCTAGCCACACCACAACCAACATCTAGAACCACTCTTAC
TTTCTTCCCCCAATCAAGAATAGGTAACGTCTATAAAAAGAAAACAGTAACTCAGTAAAA
CTGAATCAATCTTTTGGATATGTAAATATGTTTTTTTACCTTTTGTATGAAGTTGATGTA
GTGAATAACACCGTCTTTGAATTGAGTTCCACCTCCAGGGAAGACAAAGAAAGGTCCAGA
TTTACGAACCCAATTCTGATCTTTCTTGTATTCTACAAGTTTCGGATGAGGAACATTATC
ATACCAAATCTGCAGACACAAGAAAAACAAGAATTTAGAAAGCAGAGGAATCACAATCTT
CACAAGCAAGTAACAAGAAAAGATCTTACCGAAAAAATAGAAACGAGTCTCTCACCATGT
CTCTACTCTGAGGCCAAGGAAGAGGAACTTTGTAATGAGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 21-K116548-0022-356-E04C-8409
GenBank Accession LN495817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116548-0022-356-E04C-8409
TCCTCTTCCTTGGCCTCAGAGTAGAGACATGGTGAGAGACTCGTTTCTATTTTTTCGGTA
AGATCTTTTCTTGTTACTTGCTTGTGAAGATTGTGATTCCTCTGCTTTCTAAATTCTTGT
TTTTCTTGTGTCTGCAGATTTGGTATGATAATGTTCCTCATCCGAAACTTGTAGAATACA
AGAAAGATCAGAATTGGGTTCGTAAATCTGGACCTTTCTTTGTCTTCCCTGGAGGTGGAA
CTCAATTCAAAGACGGTGTTATTCACTACATCAACTTCATACAAAAGGTAAAAAAACATA
TTTACATATCCAAAAGATTGATTCAGTTTTACTGAGTTACTGTTTTCTTTTTATAGACGT
TACCTATTCTTGATTGGGGGAAGAAAGTAAGAGTGGTTCTAGATGTTGGTTGTGGTGTGG
CTAGCTTTGGTGGGACTTTACTTGATAAGAACGTTATCACAATGTCATTTGCTCCTAAAG
ACGAACACGAAGCTCAGATTCAGTTCGCACTGGAACGAGGGATTCCCGCTACACTCGCTG
TTATTGGAACTCAAAAGCTT

Last Updated on 06.12.2018 16:11