Sequences from confirmation amplicon


Confirmation sequences for line 356H02 and hit At1g04800 are shown.
Line ID 356H02
Locus At1g04800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1348982
Primers used for PCR 19lg - 8474, show primer details
   
Sequence Name 39-K113947-0022-356-H02C-19lg
GenBank Accession LN503427 [GenBank]
Sequencing primer gene specific primer, 19lg: 5'-ATCAATGCACATTACGGTTAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K113947-0022-356-H02C-19lg
GCATTCTCCACATTTCTTTACGGCTCCTCCGCCGATTCCTCCAATGGCTCCACCGTCAAC
TCCTTTACCTGCTCCACCGTCAAATCCTTTTCCGACTCCTCCCTCAATTCCTTTTCCGAT
TCCTCCCTCAAATCCTTTGCCGACTCCTCCATCAAATCCTTTTCCGGCTCCACCGTCAAC
TCCTTTGCCAACTCCTCCATCAAATCCTTTCCCGGCTCCACCGTCAACTCCTTTACCGGC
TCCACCGTCAACTCCTTTTCCGAAGCCACCGTCAACTCCTTTACCGGCTCCACCTCCGAA
TCCTCCTCCACCAAATCCACCGCCTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 11-K113946-0022-356-H02C-8409
GenBank Accession LN491200 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K113946-0022-356-H02C-8409
GCATTTACATTGATAGGCGGTGGATTTGGTGGAGGAGGATTCGGAGGTGGAGCCGGTAAA
GGAGTTGACGGTGGCTTCGGAAAAGGAGTTGACGGTGGAGCCGGTAAAGGAGTTGACGGT
GGAGCCGGGAAAGGATTTGATGGAGGAGTTGGCAAAGGAGTTGACGGTGGAGCCGGAAAA
GGATTTGATGGAGGAGTCGGCAAAGGATTTGAGGGAGGAATCGGAAAAGGAATTGAGGGA
GGAGTCGGAAAAGGATTTGACGGTGGAGCAGGTAAAGGAGTTGACGGTGGAGCCATTGGA
GGAATCGGCGGAGGAGCCGGTAAAGAAATTGGTGGAGGAATTGGCGGCGGAGGTCACTGA
GAATTTGTTTAACCGTAATGTGCATTGA

Last Updated on 06.12.2018 16:11