Sequences from confirmation amplicon


Confirmation sequences for line 357B05 and hit At5g47580 are shown.
Line ID 357B05
Locus At5g47580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19296776
Primers used for PCR NK30 - 8474, show primer details
   
Sequence Name 42-K102592-0022-357-B05C-8409
GenBank Accession LN504422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102592-0022-357-B05C-8409
ATTGTATTCTTTGCTTATAACTGAATACAGATGTTAACAACATCAGTGATGACAAGAATC
CCTAAAGCCATAAGATTCATGAGGAGATCATTGTTGTCCATAATACCCAGAGAAGGCATC
GAATTACCCATCACAGTACATACAAAGACGGTGCTGCTGAGTTTAGCTAACTGATCCTGA
CGACTCGGCATGGAAGTGTTGAGATCCACAGAGAGTTTGATTCCGACAGCGATGAAAGTC
AGAGAAGTGGCGTTGAGAGAGAAGAACTTACAGGGAAACCAGAATTTGCGGTGTCGGAAG
CCGTGTATAAGATCGGCGGCCATGGCTAGACCACAGATGAGTGAAGCTACGGCAACGTAG
ATACCAATC
   
Sequence Name 42-K102593-0022-357-B05C-NK30
GenBank Accession LN504423 [GenBank]
Sequencing primer gene specific primer, NK30: 5'-AACCCTTACCTTCGATTGGTATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102593-0022-357-B05C-NK30
GGCCGCCGATCTTATACACGGCTTCCGACACCGCAAATTCTGGTTTCCCTGTAAGTTCTT
CTCTCTCAACGCCACTTCTCTGACTTTCATCGCTGTCGGAATCAAACTCTCTGTGGATCT
CAACACTTCCATGCCGAGTCGTCAGGATCAGTTAGCTAAACTCAGCAGCACCGTCTTTGT
ATGTACTGTGATGGGTAATTCGATGCCTTCTCTGGGTATTATGGACAACAATGATCTCCT
CATGAATCTTATGGCTTTAGGGATTCTTGTCATCACTGATGTTGTTAACATCTGTATTCA
GTTATAAGCAAAGAATACAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11