Sequences from confirmation amplicon


Confirmation sequences for line 357C12 and hit At5g09830 are shown.
Line ID 357C12
Locus At5g09830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3058485
Primers used for PCR AM26 - 8474, show primer details
   
Sequence Name 09-K105747-0022-357-C12C-AM26
GenBank Accession LN490178 [GenBank]
Sequencing primer gene specific primer, AM26: 5'-AAACAATTGGAACACATGAATCAA-3'
Sequence (A. th genome BLAST matches underlined)
>09-K105747-0022-357-C12C-AM26
CTTCATCTTCACATGTTATAAAGCAAAGAAACTATACACATCCTTGGATCTGCTTTTTGA
GTTCTAGTTCACAAAAAACCATGACACAAATAAAATTTTCCGACAAGAACACTCACTACT
AAGTTGCTAATACATCAGGAACACTACGTCATCAAATCCTGAGCTCAGTATCACACTGCA
TCAAATTACACCTAACTACGCAAACAACCACCTTTTATATTCTGTTTCAGAGCTGAAAGA
CAACACATGGCTTTAATGAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 25-K105748-0022-357-C12C-8409
GenBank Accession LN497444 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>25-K105748-0022-357-C12C-8409
GCATTTACATTGAATATATCCTGATTCATTAAAGCCATGTGTTGTCTTTCAGCTCTGAAA
CAGAATATAAAAGGTGGTTGTTTGCGTAGTTAGGTGTAATTTGATGCAGTGTGATACTGA
GCTCAGGATTTGATGACGTAGTGTTCCTGATGTATTAGCAACTTAGTAGTGAGTGTTCTT
GTCGGAAAATTTTATTTGTGTCATGGTTTTTTGTGAACTAGAACTCAAAAAGCAGATCCA
AGGATGTGTATAGTTTCTTTGCTTTATAACATGTGAAGATTGAAGTTACTTCAATGTCAA
TCTATTTCCCTTTTCACTTGATTCATGTGTTCCAATTGTT

Last Updated on 06.12.2018 16:11