Sequences from confirmation amplicon


Confirmation sequences for line 357C12 and hit At5g14650 are shown.
Line ID 357C12
Locus At5g14650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4725526
Primers used for PCR CW45 - 8474, show primer details
   
Sequence Name 12-K117142-0022-357-C12C-CW45
GenBank Accession LN491762 [GenBank]
Sequencing primer gene specific primer, CW45: 5'-GTGTATACTGGAAAGGAAACCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117142-0022-357-C12C-CW45
GGACATCATGTGCTTGACATGGCGCAAAGGTGACGGGACAAGTGATGACACAAAGGTAAG
TGAGCATTTCTATTGTTTAGCTGCGTTTCTATCCGTCTCATCGATATATAATGGAACCGT
TTGTATCGCAGGCTTTTGAAGATGCTTGGCAGGTAGCTTGTAAGGTGGCAGCGTCAACAT
TATTAGTTCCATCTGGTTCTACGTTCCTTGTTGGACCTGTTTCCTTCCTGGGAAAAGAAT
GTAAAGAGAAAATCGTCTTTCAGGTAATTTACTCAAGAACAAAAAAATGTTAATCAAGCA
TTTCTAGTTATATAATCGTGACTGATGTATTTGGATACTGTGTAGTTAGAAGGAAAGATC
ATAGCTCCAACGAGTGCTAGTGCATGGGGATCAGGTCTCTTGCAATGGATAGAATTCAAA
GCACTTCAAGGAATCACTATAAAAGGAAAAGGGATCATCGATGGACGAGGATCAGTATGG
TGGAACGACATGGTAAACAGTTAATTCAATTTTCTTGGCTAAACAAAATTGTGAAAGCTT
GCAATGACATCTGCTAACGTTCTTTTTCAACTGTGACAGATGGGTACAAAAATGCCAAGG
ACCAAACCAACAGTAAGTTTTACCTGAGTAAATTACATGGATATTGCCAAATTAAAACCA
ACATTTATTTTGGATTCACCATGGTTGGTATGTAGGCTTTGAGGTTCTACGGGAGTAACG
GTGTTACGGTCAGTGGAATAACCATACAGAACAGCCCTCAAACCCACCTCAAGTTTGATA
ACTGCATAAGCATCCAAGTTTCTGATTTCACAACTTCATCCCCTGGAGATTCCCCAAACA
CAGATGGAATCCACCTGCAAAACTCTCAATTCAAAAATGTAAATAGATGAAAATCACAGC
ATACAATGGCTTCATGTCCGGGAAATCTACATGGATC
   
Sequence Name 02-K117141-0022-357-C12C-8409
GenBank Accession LN487207 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117141-0022-357-C12C-8409
TTTCATCTATTTACATTTTTGAATTGAGAGTTTTGCAGGTGGATTCCATCTGTGTTTGGG
GAATCTCCAGGGGATGAAGTTGTGAAATCAGAAACTTGGATGCTTATGCAGTTATCAAAC
TTGAGGTGGGTTTGAGGGCTGTTCTGTATGGTTATTCCACTGACCGTAACACCGTTACTC
CCGTAGAACCTCAAAGCCTACATACCAACCATGGTGAATCCAAAATAAATGTTGGTTTTA
ATTTGGCAATATCCATGTAATTTACTCAGGTAAAACTTACTGTTGGTTTGGTCCTTGGCA
TTTTTGTACCCATCTGTCACAGTTGAAAAAGAACGTTAGCAGATGTCATTGCAAGCTTTC
ACAATTTTGTTTAGCCAAGAAAATTGAATTAACTGTTTACCATGTCGTTCCACCATACTG
ATCCTCGTCCATCGATGATCCCTTTTCCTTTTATAGTGATTCCTTGAAGTGCTTTGAATT
CTATCCATTGCAAGAGACCTGATCCCCATGCACTAGCACTCGTTGGAGCTATGATCTTTC
CTTCTAACTACACAGTATCCAAATACATCAGTCACGATTATATAACTAGAAATGCTTGAT
TAACATTTTTTTGTTCTTGAGTAAATTACCTGAAAGACGATTTTCTCTTTACATTCTTTT
CCCAGGAAGGAAACAGGTCCAACAAGGAACGTAGAACCAGATGGAACTAATAATGTTGAC
GCTGCCACCTTACAAGCTACCTGCCAAGCATCTTCAAAAGCCTGCGATACAAACGGTTCC
ATTATATATCGATGAGACGGATAGAAACGCAGCTAAACAATAGAAATGCTCACTTACCTT
TGTGTCATCACTTGTCCCGTCACCTTTTGCGCCATGGTCAAGCACATTGATTGTTC

Last Updated on 06.12.2018 16:11