Sequences from confirmation amplicon


Confirmation sequences for line 357D08 and hit At4g01070 are shown.
Line ID 357D08
Locus At4g01070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:461955
Primers used for PCR 39oc - 8474, show primer details
   
Sequence Name 78-K114716-0022-357-D08C-39oc
GenBank Accession LN513726 [GenBank]
Sequencing primer gene specific primer, 39oc: 5'-AAAAAGAGGTTTTGTGATCCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K114716-0022-357-D08C-39oc
CGCACCTCCCGGGAGGATTTTTAACTCATTGTGGATGGAATTCGACTCTAGAGAGTGTAG
TAAGCGGTATTCCACTTATAGCATGGCCATTATACGCAGAACAGAAGATGAATGCGGTTT
TGTTGAGTGAAGATATTCGTGCGGCACTTAGGCCGCGTGCCGGGGACGATGGGTTAGTTA
GAAGAGAAGAGGTGGCTAGAGTGGTAAAAGGATTGATGGAAGGTGAAGAAGGCAAAGGAG
TGAGGAACAAGATGAAGGAGTTGAAGGAAGCAGCTTGTAGGGTAAATGGCTTCAATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AACGTTATT
   
Sequence Name 30-K114715-0022-357-D08C-8409
GenBank Accession LN499834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114715-0022-357-D08C-8409
ATTTACATTGAATTGAAGCCATTTACCCTACAAGCTGCTTCCTTCAACTCCTTCATCTTG
TTCCTCACTCCTTTGCCTTCTTCACCTTCCATCAATCCTTTTACCACTCTAGCCACCTCT
TCTCTTCTAACTAACCCATCGTCCCCGGCACGCGGCCTAAGTGCCGCACGAATATCTTCA
CTCAACAAAACCGCATTCATCTTCTGTTCTGCGTATAATGGCCATGCTATAAGTGGAATA
CCGCTTACTACACTCTCTAGAGTCGAATTCCATCCACAATGAGTTAAAAATCCTCCCGTG
GATGGATGCGCCAAGACTTGGGCTTGTGGAGCCCAAAAAGGGATCA

Last Updated on 06.12.2018 16:11