Sequences from confirmation amplicon


Confirmation sequences for line 357D09 and hit At2g24150 are shown.
Line ID 357D09
Locus At2g24150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10266132
Primers used for PCR 23sa - 8474, show primer details
   
Sequence Name 54-K116229-0022-357-D09C-23sa
GenBank Accession LN508592 [GenBank]
Sequencing primer gene specific primer, 23sa: 5'-TTCTTCTCTCTTGAGCTTTGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K116229-0022-357-D09C-23sa
CACATTCACATTCTCAGTTCTTACCTCACAAGGAGAAGCTATTTCCAAATGGCCGTGGCT
TCGTGTACTTAGTCGGAGCAATGGGTTGTTTAATCTGCAGCTCTGTTTCACACCTCCTTG
CTTGTCACTCAAAACGTTTCAACGTCTTCTTCTGGCGTTTGGACTACGCAGGAATATCAC
TTATGATCGTCGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTC
   
Sequence Name 14-K116228-0022-357-D09C-8409
GenBank Accession LN492661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116228-0022-357-D09C-8409
AGAAGAACGTTGAAACGTTTTGAGTGACAAGCAAGGAGGTGTGAAACAGAGCTGCAGATT
AAACAACCCATTGCTCCGACTAAGTACACGAGCCACGGCCATTTTGGAATAGCTTCTCCT
TGTGAGGTAAGACATGAGATGTGATGTGTCACGTTCGAATCCTGTTACAGAGAGAATCTT
TTAAAGCTTAGTCATGTGAATGAGTAAAGCATCATTCTTTATTCTTTCCTCAAAAGTTTA
TGTAATCTTTTCAAAAAGTTTGGTGGGTTCAAA

Last Updated on 06.12.2018 16:11