Sequences from confirmation amplicon


Confirmation sequences for line 357E02 and hit At5g50740 are shown.
Line ID 357E02
Locus At5g50740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20637008
Primers used for PCR 35qk - 8474, show primer details
   
Sequence Name 54-K115459-0022-357-E02C-35qk
GenBank Accession LN508578 [GenBank]
Sequencing primer gene specific primer, 35qk: 5'-AAATCTGGTCCAACTTATTGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115459-0022-357-E02C-35qk
GATAGTACAAATCAGGATATATGAAACATAAACCCTAATTAAAGAAGTGTTTAGAACTTT
GGGATTCTTGGGGAATAGGTGAAGTAAAAGTAAAGTAGTTGACTTAAGTTAGACTCGATT
CAACACAATTCGTATTATTTATGTAATCTTGAAACACTCAAAACAAACAAAAATATATGT
GAAGAAGAACGGTTTGTAACTATATGATTATAATAACAATAATCAGGTGAATGAGAGTTT
CTTTTTTAATGAAAGATTCACTAAACACATGCTTTTTTCAAATTGCTATGTTTTAAAAAG
GTCCTACACAGTACACAGTACTTTCAGTAAAATAAAAAGAGTTGGAATCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GC
   
Sequence Name 05-K115458-0022-357-E02C-8409
GenBank Accession LN488479 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115458-0022-357-E02C-8409
ATTTACATTGAATATATCCTGATTCCACTCTTTTTATTTTACTGAAAGTACTGTGTACTG
TGTAGGACCTTTTTAAAACATAGCAATTTGAAAAAAGCATGTGTTTAGTGAATCTTTCAT
TAAAAAAGAAACTCTCATTCACCTGATTATTGTTATTATAATCATATAGTTACAAACCGT
TCTTCTTCACATATATTTTTGTTTGTTTTGAGTGTTTCAAGATTACATAAATAATACGAA
TTGTGTTGAATCGAGTCTAACTTAAGTCAACTACTTTACTTTTACTTCACCTATTCCCCA
AGAATCCCAAAGTTCTAAACACTTCTTTAATTAGGGTTTATGTTTCATATATCCTGATTT
TGTACTTATCTGAAATCAAATTTATATTTATAATGCCATCAATAAGTTGGACCAGATT

Last Updated on 06.12.2018 16:11