Sequences from confirmation amplicon


Confirmation sequences for line 357G03 and hit At3g56680 are shown.
Line ID 357G03
Locus At3g56680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20991326
Primers used for PCR EP35 - 8474, show primer details
   
Sequence Name 35-K103228-0022-357-G03C-EP35
GenBank Accession LN501792 [GenBank]
Sequencing primer gene specific primer, EP35: 5'-AGACTGTATATTTGACGGTTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103228-0022-357-G03C-EP35
CGTTACATTGACGTCGTTCGTTCCCCTCGCCGTCAAGCCGTGAACCACCACCAAATCTTT
GTGCTACCGTCAAAGCTTCACGTCTACCGTCGAAGCTGCAAATCCACTATCTAAGCCATA
TATCTCTGAATGTCAAAAGAAACTTAAAATTGAATCAGAGATGGGGATGCATTTTTTAGA
AGATAAACGCATTCTTTTCACCTCATTCTCAACTCCNGCTCATAGTTTAAGGGCAAAAAT
GTCCGTAACAATGAAAATTGGAGTTATTATATGCTTTTACTGTTGTTGTTTTTTACGGCA
AAATAAGGTCATCCATTTAAGCTAAAAACGGGTCGGTTTTCTTCGAGAGAAAACTAGTTC
GGAGTAGAAGAACTTACATGGGAAAAAATAGAATCTCGTGCTCTTGCCTTCTTACCAAGA
CAATCAGCACGGACATTGTTTAGAGTCTCTGTTTTGAAAGAAATGTTTTCAAAATGCTTG
TTTAGAATATAAGCTCTATATNGTATACCTACACATGTTATCGTGGGTCCAAATAGAAAA
GAAAAACTATATTTAAAAAATTAAAATTATATGAGATATTTATTTTCAATGAANAGGAGA
AAAATAAAAAATAAAGAAGGAAAAAATATAANGTTTCCTTTCCCTCTCGTTCGTTGGCTT
CATGTCCGGGAAATGTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTA
   
Sequence Name 03-K103229-0022-357-G03C-8409
GenBank Accession LN487446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103229-0022-357-G03C-8409
CCGGAATGAGCCACGAAGAGAGGGAAAGGAAACTTTATATTTTTTCCTTCTTTATTTTTT
ATTTTTCTCCTTTTCATTGAAAATAAATATCTCATATAATTTTAATTTTTTAAATATAGT
TTTTCTCTTCTATTTGGACCCACGATAACATGTGTAGGTATACTATATAGAGCTTATATT
CTAAACAAGCATTTTGAAAACATTTCTTTCAAAACAGAGACTCTAAACAATGTCCGTGCT
GATTGTCTTGGTAAGAAGGCAAGAGCACGAGATTCTATTTTTTCCCATGTAAGTTCTTCT
ACTCCGAACTAGTTTTCTCTCGAAGAAAACCGACCCGTTTTTAGCTTAAATGGATGACCT
TATTTTGCCGTAAAAAACAACAACAGTAAAAGCATATAAAAACTCCNATTTTCATTGTTA
CGGACATTTTTGCCCTTAAACTATGAGCTGGAGTTGAGAATGAGGTGAAAAGAATGCGTT
TATCTTCTAAAAAATGCATCCCCATCTCTAATTCAATTTTAAGTTTCTTTTGACATTCAG
AGATNTATGGCTTAGATNGTGGATTTGCAGCTTCGACGGTNGACGTGAAGCTTTGACGGT
NGCACAAAGATTTGGTGGTGGTTCACGGCTTTGACGGCAAGGGGTAACGAACGACGTCNA
TGGTAAACGCTATACCCTAGAANTAGACTTGTAAACCCNAACCGTCAA

Last Updated on 06.12.2018 16:11