Sequences from confirmation amplicon


Confirmation sequences for line 358G08 and hit At4g13950 are shown.
Line ID 358G08
Locus At4g13950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8058281
Primers used for PCR BV79 - 8474, show primer details
   
Sequence Name 45-K106149-0022-358-G08C-BV79
GenBank Accession LN505537 [GenBank]
Sequencing primer gene specific primer, BV79: 5'-CTGAGAATTTCCAGGACTTATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K106149-0022-358-G08C-BV79
ATGCTTCCAGATTTTCCTTTGCTCGTGTATTAGCAAAAATCTCAGAATCCTAAGTGAAAA
GATTCTAATTTGGTCCAAAAGCACCCAAAAACAAGCAAACAAACACCCAAATTTATCAAT
CAATCTACCAAAAAAACTTAATATAATTTATAGATCTCAATAATTGAACATTGATAACCA
ACTAGTGGCAGAGTTACATATATTATAACAATTCTTATGTCAAAAACACATATATAACAA
CAGCAATGCAAAAGCTTAATCATATAATTTACTTGCCTCGGCCAAAAAGCTTCCAATTTT
GGCAATTTCTTGGATCTTACCTTCATGTGTTGATGGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 61-K106150-0022-358-G08C-8409
GenBank Accession LN510526 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K106150-0022-358-G08C-8409
CCATTTACATCAACACATGAAGGTAAGATCCAAGAAATTGCCAAAATTGGAAGCTTTTTG
GCCGAGGCAAGTAAATTATATGATTAAGCTTTTGCATTGCTGTTGTTATATATGTGTTTT
TGACATAAGAATTGTTATAATATATGTAACTCTGCCACTAGTTGGTTATCAATGTTCAAT
TATTGAGATCTATAAATTATATTAAGTTTTTTTGGTAGATTGATTGATAAATTTGGGTGT
TTGTTTGCTTGTTTTTGGGTGCTTTTGGACCAAATTAGAATCTTTTCACTTAGGATTCTG
AGATTTTTGCTAATTACACGAGCAAAGGAAAAATCGTGAGAAAGCAAATTAAACTTGCAA
TAAGTCCTGGAAATTCTCA

Last Updated on 06.12.2018 16:11