Sequences from confirmation amplicon


Confirmation sequences for line 358H04 and hit At4g15620 are shown.
Line ID 358H04
Locus At4g15620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8914919
Primers used for PCR 24sj - 8474, show primer details
   
Sequence Name 43-K116249-0022-358-H04C-24sj
GenBank Accession LN504964 [GenBank]
Sequencing primer gene specific primer, 24sj: 5'-AAGCCACCATCACTAGATCTCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116249-0022-358-H04C-24sj
TCTTCCCTGCTGATCATCAGAATCGCTATCGAGATCGCCGTGTAACCGCAAGCTATAGCG
TTCACAGAAATGTTGTACCTACAACATTTGTAATACCACATTGCTTATTTCCGTATTTTA
TGCATGTTCTAATGTAATTCTCTTCTCTTTTTTTGCCATGAAAGAGTAAAAATAATTAAA
AATCAGATTAGCATTTAATTTGAATTCATGTTTCTAGTTTATAAAATTTATTTAGATATG
TAGGCTATACACTTTTGGTCATGCCAGCTAAGAAGTAAGAACTGTTGGACACTAAATTTA
AAGTAACATAAGAGCTGGAAAAATGTCAGGTAAAAATTGGCTATGGGCCAACATTGCTGT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K116248-0022-358-H04C-8409
GenBank Accession LN494913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116248-0022-358-H04C-8409
CATTTCACAGCAATGTTGGCCCATAGCCAATTTTTACCTGACATTTTTCCAGCTCTTATG
TTACTTTAAATTTAGTGTCCAACAGTTCTTACTTCTTAGCTGGCATGACCAAAAGTGTAT
AGCCTACATATCTAAATAAATTTTATAAACTAGAAACATGAATTCAAATTAAATGCTAAT
CTGATTTTTAATTATTTTTACTCTTTCATGGCAAAAAAAGAGAAGAGAATTACATTAGAA
CATGCATAAAATACGGAAATAAGCAATGTGGTATTACAAATGTTGTAGGTACAACATTTC
TGTGAACGCTATAGCTTGCGGTTACACGGCGATCTCGATAGCGATTCTGATGATCAGCAG
GGGAAGAAGAAGCAAGAAGTTGTTAATGGTGGTTTTGCTAGGAGATCTAGTGTGGGTGGC
TT

Last Updated on 06.12.2018 16:11