Sequences from confirmation amplicon


Confirmation sequences for line 359A07 and hit At5g03820 are shown.
Line ID 359A07
Locus At5g03820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1016918
Primers used for PCR PC81 - 8474, show primer details
   
Sequence Name 12-K103845-0022-359-A07C-PC81
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, PC81: 5'-GTTTGACTAAACCCAACGAATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103845-0022-359-A07C-PC81
AAAAAAAAAAACCTTTTAAAAAGGGGTCCCCAAAATTTTTTTAAACCCAAAAAAAAAAAA
CCCTTTATTTTTTTTGGGGGGATTGCCAAAAAAAGGGGAAGGGGGCCCAAACCCCCCTGG
GGGAAAAATTGGGGGTTAAGGGCCCCCCTTTTTAATTTCCAAAAAAAATTTTCCCCCAAA
AGGGGGAACTTTAAAAAAAAAAAAGGGATTTTCCTTAATTTTTTTCCCTTAAAAACCCTT
TTTTTCCCCCCCCCCCCTTTTTTTTTTTTTAAAACTTTTTTTCAAAAAAAAAAAAAAAAT
TTTTTTAAAAAGGTTAAAAACCTTTTGGGTTTACCCCGGTTTTCCCCCCCGGTTTGGGAC
CGGGAACCCCTTTTTCCCGGCCTTTAAAAAAAGGGGAAATTTTTTTTTTAATCCCGGGAA
AAAAACCCCCCCAAAAACCCTTAAAAAAACCATTTTTCCCCCCCGGGGGGGGGATTTTTA
ACCCCAAAGGGCCCTGGGGTTTTTTTTTAAAGAAAAATTTGCCCAAAAATTTTCGGGTTC
AAACCCCCCCAATTCCATTTTTTTTTTTTGGAAAGACCCCCCCATTTTTTAAATTTCCTT
TAATTCCCCAAGGCTTTTTTTTTTTAAAAAGAAAATTTCCCTTTTGAAAGGGCTCCCGTC
CCGGGAAATCCCCGGGGAACCCCCTGGGAAAAAAGGGTTAATTGGGAAAAAAAAAAAGAA
AATTTCCCCTTTTTTTTTCCTTTCAAAAATTT
   
Sequence Name 28-K103846-0022-359-A07C-8409
GenBank Accession LN498812 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103846-0022-359-A07C-8409
CGGACATGAGCCTTTAAATTGAATATATCCTGATCAACAAACAAAAGCCTAAGGAAATTA
AATGAAATGATATATATAGATGGTACGTACTATAACGTAAATAGTGAATTAGGCTGGTTC
GAACCGGTAAAGTCTGTGGCAAGTTTTCCGTTAGAGAAACGGCCAGTGGCATTGTGGGCT
AAGAAGTCACGTCCATAGGGAGGAAAATTGGCTTTGATGAGGGTATTGAGGCGGTTGTTG
TTGCCGGCATCGACAACTGAATCTCCCATTATGATTAGAGCCGGTACAAGTGGCTCACCG
GTCCCAACGCCGGCGTAGAAACAGGCGATAACCGAAAAGGTCATCAACATTATAATAAAC
ATCTTCATTTTCTGTTTGAAATAATGTAGTAAAAGATAGAGATAGGAGTGAGTGAAGAGA
TAGGGTTTTAAAGGAAGATAGATTAAGGAAATTACATTCATTTGTTTGAAGCTTGCATGC
TTCGGTAGTAACGTCGTATTGGAATTTAAAGATGGTAGACCTTAACACGCANTTCATCCA
CAAGAGGACATACGACACCTCTNACATTTTTCATGCAATCACAACAAACAAATTAAAGGA
TTTTTTTTTTTTGGGTTTTAAAACTTTTTCGGGACCCATTTGTAAAATGGGTTTTTTTTT
TTTTTTTTTTTTAAAAAATTTTAACCCTCCGGGGG

Last Updated on 06.12.2018 16:11