Sequences from confirmation amplicon


Confirmation sequences for line 359F04 and hit At1g29240 are shown.
Line ID 359F04
Locus At1g29240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10218406
Primers used for PCR 16ve - 8474, show primer details
   
Sequence Name 72-K117997-0022-359-F04C-16ve
GenBank Accession LN512797 [GenBank]
Sequencing primer gene specific primer, 16ve: 5'-TTCAGAGTTGAACTTCTTCCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K117997-0022-359-F04C-16ve
GACTTTCCCTCACTTGTGCTTAGAAACTGAATCAAGAGCTAGCTGGAAAATGTACAAGAG
CTACTAGTCAGTGAATGTCTAATCATATTTATAAGGGACTAATAGGAGTCTAAAGGTGGA
ACCTTTTTAACAGATTGGAATCGATATTTGAGTTGAGCAACCTTGCTTGACTTCACTTGA
TCATGACTTGGTGATGTTATTGGAGAGTTGTGTTTAGCTTTAAAACCAGGCACTGTGTTC
AGCATGCCGAGAGAGTCTTTAAAGCACAATTGTGGCAACATTCCACAACCTCTCTTTGAA
AGATAGGCGTACTCAGAAACTTCATCATCTTCCTCACCCACCTTGCTTTCTTCATCATCA
ATGTCTTGGTGGTAATAGTAAGATGGGACTATAGACACATCATATCTATTAGGAGTTTGC
TGCTTCTCCCCCGGTACTAACTCTCTTATCTGTATCGTTGGTTCAGCCATAAAAGTGGAT
GGTTTTCGATTCGAAGCATAATGAGACTGCTCCACAGTCATAGCTTTGGCAGCTGGTAAA
AAACGACTCATCATGAAATCCAGTGGAACCCGACAACAATGACAACAGTGTCCACAGCCA
TGTAGACACGTCGTTGGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 32-K117996-0022-359-F04C-8409
GenBank Accession LN500751 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117996-0022-359-F04C-8409
ATTGAATATATCCTGGCCAACGACGTGTCTACATGGCTGTGGACACTGTTGTCATTGTTG
TCGGGTTCCACTGGATTTCATGATGAGTCGTTTTTTACCAGCTGCCAAAGCTATGACTGT
GGAGCAGTCTCATTATGCTTCGAATCGAAAACCATCCACTTTTATGGCTGAACCAACGAT
ACAGATAAGAGAGTTAGTACCGGGGGAGAAGCAGCAAACTCCTAATAGATATGATGTGTC
TATAGTCCCATCTTACTATTACCACCAAGACATTGATGATGAAGAAAGCAAGGTGGGTGA
GGAAGATGATGAAGTTTCTGAGTACGCCTATCTTTCAAAGAGAGGTTGTGGAATGTTGCC
ACAATTGTGCTTTAAAGACTCTCTCGGCATGCTGAACACAGTGCCTGGTTTTAAAGCTAA
ACACAACTCTCCAATAACATCACCAAGTCATGATCAAGTGAAGTCAAGCAAGGTTGCTCA
ACTCAAATATCGATTCCAATCTGTTAAAAAGGTTCCACCTTTAGACTCCTATTAGTCCCT
TATAAATATGATTAGACACTCACTGACTAGTAGCTCTTGTACATTTTCCAGCTAGCTCTT
GATTCAGTTTCTAAGCACAAGTTGAGTGGTAAAGTTCATTCACCAGTTCATCCAAGCAAT
GGGAAGAAGTTCAACTCTGA

Last Updated on 06.12.2018 16:11