Sequences from confirmation amplicon


Confirmation sequences for line 360C11 and hit At4g26330 are shown.
Line ID 360C11
Locus At4g26330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13320871
Primers used for PCR CR26 - 8474, show primer details
   
Sequence Name 19-K019984-022-360-C11C-CR26
GenBank Accession LN494567 [GenBank]
Sequencing primer gene specific primer, CR26: 5'-ATTTAAGGGGAGTACCGTGAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K019984-022-360-C11C-CR26
TACCGTCGATAACAATTCTGGTGGGAAAACTCCGATCAACTGTCGAAGCTGCGACAGAAA
CCGCCCAAGGAGCGACATTCTGAACAACACCAGGATCAGGGCCATCGTTTCCGGTAGAGA
ACACCACGCTGATTCCTCTCTCCGCTGCGTGAAAAGCCCCGATGTCTGCGCTCGACTCGA
AAAACGGAGATAACGGCGGCGAATAGCCAAAAGAGGCCGAAATCACGTGGACGCCGTCGT
GGATGGCGTCGTCAAAAGCTGCTAAGATATCAGCCTCCGTGCAAACACCTTCCAGATCCT
TCCCCCAACACGTCTTGAACACAGCTAGCCTAGCCAATGGAGCTCCGCCGCGAGCAGTGC
CGCGTCCAAGACCGAAGAAGCCGGAGACATTGCGAACAACGGATCCGACGGCGGTGGAAG
CTGTGTGTGTGCCGTGACCGAGGTAATCTCGCGGCGATCGGTATTCAGGATCTCGCGTAA
AGTCAATTGTTCCGTACGTCTCTTCGAATCCTCTGAGGTAAAATCGAGCACCGATCAATT
TACGATTGCAATGTACAGATGGATCAAAGTCTTCCCCTCCGACGCATTTTCCGTTCCATG
ACGAAGGTATAGGTTTCGCCTCCGGCGTTTCCCTGAAACTCTCCGATTCCGGCCAGATTC
CTAAAATTGAAAGTAACAAAAGTTTCAATGAAATGCTCAAAATGAGAGCCCTGAAACAAA
GAAGAGTCGGGAAATAAACAA
   
Sequence Name 20-K019984-022-360-C11C-8409
GenBank Accession LN495021 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K019984-022-360-C11C-8409
TTACTTTTTCTTTTTCATTACATGAAGCCATTTTATACACATTATATATCCTCGGAATCT
TCGATACTGGTCTCTTGTTTATTCCCGACTCTTCTTTGTTTCAGGGCTCTCATTTTGAGC
ATTTCATTGAAACTTTTGTTACTTTCAATTTTAGGAATCTGGCCGGAATCGGAGAGTTTC
AGGGAAACGCCGGAGGCGAAACCTATACCTTCGTCATGGAACGGAAAATGCGTCGGAGGG
GAAGACTTTGATCCATCTGTACATTGCAATCGTAAATTGATCGGTGCTCGATTTTACCTC
AGAGGATTCGAAGAGACGTACGGAACAATTGACTTTACGCGAGATCCTGAATACCGATCG
CCGCGAGATTACCTCGGTCACGGCACACACACAGCTTCCACCGCCGTCGGATCCGTTGTT
CGCAATGTCTCCGGCTTCTTCGGTCTTGGACGCGGCACTGCTCGCGGCGGAGCTCCATTG
GCTAGGCTAGCTGTGTTCAAGACGTGTTGGGGGAAGGATCTGGAAGGTGTTTGCACGGAG
GCTGATATCTTAGCAGCTTTTGACGACGCCATCCACGACGGCGTCCACGTGATTTCGGCC
TCTTTTGGCTATTCGCCGCCGTTATCTCCGTTTTTCGAGTCGAGCGCAGACATCGGGGCT
TTTCACGCAGCGGAGAGAGGAATCAGCGTGGTGTTCTCTACCGGA

Last Updated on 06.12.2018 16:11