Sequences from confirmation amplicon


Confirmation sequences for line 360H12 and hit At4g25920 are shown.
Line ID 360H12
Locus At4g25920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13166001
Primers used for PCR 19fp - 8474, show primer details
   
Sequence Name 59-K111394-0022-360-H12C-19fp
GenBank Accession LN510033 [GenBank]
Sequencing primer gene specific primer, 19fp: 5'-ATGCAAAGATCTCCAATGTCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K111394-0022-360-H12C-19fp
ACTCGGTTCTCTCCCTCTTTAAACACCATGAACTTTTTTGTTACATTATACAGTGTGGTG
TTGTCAATGTCCTCTAAATCCTCTCCATACCTGCAAAGACATGATAAAGCGCGCCAATAT
GATACTCAAGCAAAGGATTAGAGATTTTGGTATATATGTTATGAAGACAAAAGCTATATA
TAGCTTTAATTAGATAGTATACCACTTGACTAGGAAAAGTTGGCCGGTGGGTGACTCCAC
AAGGTGGTCTGTCCTAGAACATGTACTCACGTCGGCCAACTCTTGAAACACCGATTTTGG
AAGATCGTCAAACTCCAATTCCACGAAATCAATTAGGTCGTCGTCCTCTGAGTGAGGATC
CAAATAGCACAAGATGTTGCCTCCGGGAGTTGGAACGTAGAACTTTTTCTCTTTCTTGGA
AAACATGATGCTCGATGAAGGGTTTATATTCTGAGGCTTGGTTTGGATGTTGATCCATTT
ACGGCTGCCAAAAGGCCTGCACATACTCAACTGAGATCCCAATAACTTGACAACAACTAC
CCAATTTTTGTCTCGTTTAGGTAAAGAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATAACCGCACGGTTATTA
   
Sequence Name 43-K111393-0022-360-H12C-8409
GenBank Accession LN504878 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K111393-0022-360-H12C-8409
ATTTCATTGAATATATCCTCTTTACCTAAACGAGACAAAAATTGGGTAGTTGTTGTCAAG
TTATTGGGATCTCAGTTGAGTATGTGCAGGCCTTTTGGCAGCCGTAAATGGATCAACATC
CAAACCAAGCCTCAGAATATAAACCCTTCATCGAGCATCATGTTTTCCAAGAAAGAGAAA
AAGTTCTACGTTCCAACTCCCGGAGGCAACATCTTGTGCTATTTGGATCCTCACTCAGAG
GACGACGACCTAATTGATTTCGTGGAATTGGAGTTTGACGATCTTCCAAAATCGGTGTTT
CAAGAGTTGGCCGACGTGAGTACATGTTCTAGGACAGACCACCTTGTGGAGTCACCCACC
GGCCAACTTTTCCTAGTCAAGTGGTATACTATCTAATTAAAGCTATATATAGCTTTTGTC
TTCATAACATATATACCAAAATCTCTAATCCTTTGCTTGAGTATCATATTGGCGCGCTTT
ATCATGTCTTTGCAGGTATGGAGAGGATTTAGAGGACATTGACAACACCACACTGTATAA
TGTAACAAAAAAGTTCATGGTGTTTAAAGAGGGAGAGAAACCGAGTTGTGTCTACACTAA
GAAGATGATCTATACAGAAGACATTGGAACCNTTTGCAT

Last Updated on 06.12.2018 16:11