Sequences from confirmation amplicon


Confirmation sequences for line 361C06 and hit At5g17980 are shown.
Line ID 361C06
Locus At5g17980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5954924
Primers used for PCR KU78 - 8474, show primer details
   
Sequence Name 30-K103307-0022-361-C06C-8409
GenBank Accession LN499678 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103307-0022-361-C06C-8409
GCCATTTACATTGAATATATCCTGACAGGAACTCAAGCAGACGAATCATTCCCCGACGCG
TGGAAAACTGATACAGCCGGAAACGTGACCGCACGAGCGAAGGTTTACATGTCGTCGAAG
CTGTGGTATCTACGCGCTACCGTGATAGAAGCACAAGACTTGTTACCGCCTCAGTTAACG
GCGTTTAAGGAAGCGTCATTTCAGTTAAAAGCTCAGTTAGGTTCCCAAGTTCAGAAAACC
AAATCCGCCGTTACTCGTAACGGAGCTCCGTCATGGAACGAAGATCTACTCTTCGTAGCC
GCCGAACCTTTCTCCGATCAGCTTGTTTTCACGTTGGAGTATCGAACATCGAAAGGACCA
GTCACCGTCGGAATGGCTCGCGTGCCACTCAGCGCAATCGAGAGACGCGTCGACAATNGC
TTGGTTGCTTCGAGATGGTTAGGATTGGAAGATCCAAACGACGAGNAGAGAGGAAACAGA
TCTNGAGTTCACATTAGGCTTTGCTTCGACGGAGGTTATCACGTGATGGACGAGGCGGCG
CANGTGTGCAGTGATTACCGACCTACGGCGAGGCAGTTATGGAAACCAGCGGTGGGAATC
GTCGAGCTCGGTATAATCGGTTGCAAAAATCTATAACCGATAAAGACGGTNAANGGAGAA
GGATCAACGGACGCGTACACGGTGGCAAANTACGGTNCTNAATGGGTCCGTACCCGAACC
GTGTCGGATTCTTTAGATCCAAANTGGAACGAANAGTACACGTGGAANGTATACAATCCG
TGTA
   
Sequence Name 06-K103308-0022-361-C06C-KU78
GenBank Accession LN488771 [GenBank]
Sequencing primer gene specific primer, KU78: 5'-CGAAGACTCCTATAGTCAAAACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103308-0022-361-C06C-KU78
TTCGTTCCATTTCGGATCTAAGAATCCGACACGGTTCGGGTACGGACCCATTTAGAACCG
TATTTCGCCACCGTGTACGCGTCCGTTGATCCTTTTCCGTTAACCGTCTTCATCGGTAAT
AGATTTTTGCAACCGATTATACCGAGCTCGACGATTCCCACCGCTGGTTTCCATAACTGC
CTCGCCGTAGGTCGGTAATCACTGCACACGTGCGCCGCCTCGTCCATCACGTGATAACCT
CCGTCGAAGCAAAGCCTAATGTGAACTCTAGATCTGTTTCCTCTCTTCTCGTCGTTTGGA
TCTTCCAATCCTAACCATCTCGAAGCAACCAAGCGATCGTCGACGCGTCTCTCGATTGCG
CTGAGTGGCACGCGAGCCATTCCGACGGTGACTGGTCCTTTCGATGTTCGATACTCCAAC
GTGAAAACAAGCTGATCGGAGAAAGGTTCGGCGGCTACNAAGAGTAGATCTTCGTTCCAT
GACGGAGCTCCGTTACGAGTAACGGCGGATTTGGTTTTCTGAACTTGGGAACCTAACTGA
GCTTTTAACTGAAATGACGCTNCCTTAAACGCCGTTNACTGAGGCGGTAACAAGTCTTGT
GCTTCTATCACGGTAGCGCGTAGATACCACAGCTTCGACGACNTGTAAACCTTCGCTCGT
GCGGTCACGTTTCCGGCTGTATCAGTTTTCCACGCGTCGGGAAATGATTCGTCTGCTTGA
GTTCCTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCANAAATGTAG

Last Updated on 06.12.2018 16:11