Sequences from confirmation amplicon


Confirmation sequences for line 361H06 and hit At2g36380 are shown.
Line ID 361H06
Locus At2g36380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15257781
Primers used for PCR 07iv - 8474, show primer details
   
Sequence Name 76-K113020-0022-361-H06C-07iv
GenBank Accession LN513428 [GenBank]
Sequencing primer gene specific primer, 07iv: 5'-TCTCACTCCAGAGTTTCTCTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113020-0022-361-H06C-07iv
GTCTCGACATTCTTAGTAAACCGGACGACTCAGCGGCGATGTTAGGACGAGATGAAGATC
TGGTCCGAACAATGAGCGGGAGAGGAAGTTTAGGCTCCACAAGTCACCGGAGTTTAGCCG
GAGCTGCTTCAAAAAGCTTCAGAGATGTGTTTGCTCCCCCAACGGACGACGTGTTTGGTC
GGAGCGATAGACGAGAAGAAGATGACGTGGAGCTCCGATGGGCGGCGCTTGAGAGATCAA
ACTATGGCTTATATTCGTTGGCTTCATGTTGATCAAACATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 44-K113019-0022-361-H06C-8409
GenBank Accession LN505243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113019-0022-361-H06C-8409
GAATATATCCTGATGTTTGATCAACATGAAGCCAACGAATATAAGCCATAGTTTGATCTC
TCAAGCGCCGCCCATCGGAGCTCCACGTCATCTTCTTCTCGTCTATCGCTCCGACCAAAC
ACGTCGTCCGTTGGGGGAGCAAACACATCTCTGAAGCTTTTTGAAGCAGCTCCGGCTAAA
CTCCGGTGACTTGTGGAGCCTAAACTTCCTCTCCCGCTCATTGTTCGGACCAGATCTTCA
TCTCGTCCTAACATCGCCGCTGAGTCGTCCGGTTTTACTAATGAATGTTCGAGACGATTT
AGTTTCCGGCGACCATTTAAAGGGGAGAGAGAAATCGNGGAGTGAGA

Last Updated on 06.12.2018 16:11