Sequences from confirmation amplicon


Confirmation sequences for line 362B11 and hit At5g41060 are shown.
Line ID 362B11
Locus At5g41060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16435989
Primers used for PCR 46pm - 8474, show primer details
   
Sequence Name 24-K115349-0022-362-B11C-46pm
GenBank Accession LN497121 [GenBank]
Sequencing primer gene specific primer, 46pm: 5'-AATTTCTTCAACGGAGACTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115349-0022-362-B11C-46pm
GTTTAGTGTTTCCCCTTTGCTTACATTTGAAGAAATCGAGAGCAAGCGATAGAAAGTGAG
AGAGAGAGATGTATGTGGTGACGCCTCCTCAAAGATCCGGTTTTGGATCCGATTGTGATT
TACGGGTTTACCAAACATGGAAAGGAAGCAATGTGAGTTTTAATGTGAAAGCTTTAGGTT
TTGGAATTACTTGCTTCTGTGTTTATACTTGAGCTAGTTTTCTGTTATTTTTCCTATTAT
TGTGTATAAATTAATTATTAATCCGTTCATGGGCTCAGTTTACTCTGTTTCCAATCTTGT
AAAGCTTAAAAATTGGGACTTGGGTTTGTGTTAGAAGTGTAGCTTTCTGGGTAGTTGTCT
ACATTTAATCATGAAATCTTTACCTTTAATCAATACCTTAGGACATTATCTGAATATGCT
TGCTACATTTATAGGAATGCTTCTTCATACAAAGGTTATATAGTTGATGCTGTCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACAATTTATA
   
Sequence Name 08-K115348-0022-362-B11C-8409
GenBank Accession LN489859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115348-0022-362-B11C-8409
TACCTTTGTATGAGAGCATTCCTATAAATGTAGCAAGCATATTCAGATAATGTCCTAAGG
TATTGATTAAAGGTAAAGATTTCATGATTAAATGTAGACAACTACCCAGAAAGCTACACT
TCTAACACAAACCCAAGTCCCAATTTTTAAGCTTTACAAGATTGGAAACAGAGTAAACTG
AGCCCATGAACGGATTAATAATTAATTTATACACAATAATAGGAAAAATAACAGAAAACT
AGCTCAAGTATAAACACAGAAGCAAGTAATTCCAAAACCTAAAGCTTTCACATTAAAACT
CACATTGCTTCCTTTCCATGTTTGGTAAACCCGTAAATCACAATCGGATCCAAAACCGGA
TCTTTGAGGAGGCGTCACCACATACATCTCTCTCTCTCACTTTCTATCGCTTGCTCTCGA
TTTCTTCAAATGTAAGCAAAGGGGAAAACACTAAAACGAAGATNACGAAAGAAGAAAAGA
GAAAGCTTTTCAGTCTCCGTGAAA

Last Updated on 06.12.2018 16:11