Sequences from confirmation amplicon


Confirmation sequences for line 362C09 and hit At3g48730 are shown.
Line ID 362C09
Locus At3g48730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18049807
Primers used for PCR BV84 - 8474, show primer details
   
Sequence Name 76-K105358-0022-362-C09C-BV84
GenBank Accession LN513401 [GenBank]
Sequencing primer gene specific primer, BV84: 5'-TCAAAACTGGAAAGAAACAAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K105358-0022-362-C09C-BV84
CCCCAAGATCCAACATAGTCAATGTATTCATTTCCATCAATGTCTCTTATTCGTGAGCCC
TTTGCAGAATCCATCACCACTGGGTTGTCCACCAACAGATTTGAAAGCACGTACAGGTGA
GTTCACACCTCCAGGCATTAAGTTCTACACAAAGGAACCAAACCAATTTCAATAATCAGA
ACAGAATGAAACCAATAAAAACAAAAACTTTATCTCAGATTATGATTCTATCTCAAGTAA
TCTTATTTTGTTAGCAATGTTCCTTGAAGGGTAACAAAGATAATCACTTTAACCAAAAAT
ATGATCAAATCCCACAAAACCAAAGCAACAGAACATCAAGGCGTAATCAATCTGAAGAAA
CATTTCTTATTGACTGAATTCATGGAAATTGAAAAGCCTAACCTTCGCAGCATTGAAAGC
TTCCTCAGATTTCTGAAGAGTGAATTTCTTCGTTGTAAATGGCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 60-K105359-0022-362-C09C-8409
GenBank Accession LN510258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K105359-0022-362-C09C-8409
TTACATTGAATATATCCTGCCATTTACAACGAAGAAATTCACTCTTCAGAAATCTGAGGA
AGCTTTCAATGCTGCGAAGGTTAGGCTTTTCAATTTCCATGAATTCAGTCAATAAGAAAT
GTTTCTTCAGATTGATTACGCCTTGATGTTCTGTTGCTTTGGTTTTGTGGGATTTGATCA
TATTTTTGGTTAAAGTGATTATCTTTGTTACCCTTCAAGGAACATTGCTAACAAAATAAG
ATTACTTGAGATAGAATCATAATCTGAGATAAAGTTTTTGTTTTTATTGGTTTCATTCTG
TTCTGATTATTGAAATTGGTTTGGTTCCTTTGTGTAGAACTTAATGCCTGGAGGTGTGAA
CTCACCTGTACGTGCTTTCAAATCTGTTGGTGGACAACCAGTGGTGATGGATTCTGCAAA
GGGCTCACGAATAAGAGACATTGATGGAAATGAATACATTGACTATGTTGGATCTTGGGG
ACCTGCTATAATTGGTCATGCTGATGATGAGGTTTGTTTCTTTCCAAGTTTTG
Primers used for PCR AP22 - 8474, show primer details
   
Sequence Name 85-K118835-0022-362-C09C-AP22
GenBank Accession LN514593 [GenBank]
Sequencing primer gene specific primer, AP22: 5'-TGGAAGTGTTTGAAGAGCTTATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K118835-0022-362-C09C-AP22
TTTCTATCAATTATGATATATTCTACCAAATTATAAGCAAAGGAAAATTATAATCTAGTC
ATTATTTATACATGTTATATTTGAATTCACGTTTGTATCCATTACAAAAATTTATGTAAT
TCGCAGAAAATATATATATGTCAAATTAAGTTATCGGACCGGATCGGGTCAAAGAAACCG
GATTGGTTAACAAAGTCAAACAAACAAATATTGACTTGAGTTTCAGATTCCAGTAAAATC
AAACCGGATCGGGTCTGAATTTAAACCACCGGGTCGGCTCCATCTAAAATCTGAAGATCA
TCTCCGCCTTATCTTGTTGACTATCTCTGTACTACTTACGAGCGCAAGTGAGAGAGTAAC
AGAGAAACGTAGAGATATAGCAAAATGGCTGCGACGCTTACTGGATCAGGGATTGCTCTA
GGGTTTTCGTGCTCCGCAAAGTTCTCTAAGAGAGCTTCTTCGTCGTCCAACCGTCGCTGC
ATCAAGATGTCGGTTTCGAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGC
   
Sequence Name 61-K118834-0022-362-C09C-8409
GenBank Accession LN510630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K118834-0022-362-C09C-8409
GCCATTTCTTCGAACCGACATCTTGATGCAGCGACGGTTGGACGACGAAGAAGCTCTCTT
AGAGAACTTTGCGGAGCACGAAAACCCTAGAGCAATCCCTGATCCAGTAAGCGTCGCAGC
CATTTTGCTATATCTCTACGTTTCTCTGTTACTCTCTCACTTGCGCTCGTAAGTAGTACA
GAGATAGTCAACAAGATAAGGCGGAGATGATCTTCAGATTTTAGATGGAGCCGACCCGGT
GGTTTAAATTCAGACCCGATCCGGTTTGATTTTACTGGAATCTGAAACTCAAGTCAATAT
TTGTTTGTTTGACTTTGTTAACCAATCCGGTTTCTTTGACCCGATCCGGTCCGATAACTT
AATTTGACATATATATATTTTCTGCGAATTACATAAATTTTTGTAATGGATACAAACGTG
AATTCAAATATAACATGTATAAATAATGACTAGATTATAATTTTCCTTTGCTTATAATTT
GGTAGAATATATCATAATTTGATTATGAAAATGTATGTTCTTTTTCAGATCTTCATAAGC
TCTTCAAACACTTCC

Last Updated on 06.12.2018 16:11