Genotyping and primer details


Genotyping and primer details for 362C09 - At3g48730
Line ID 362C09
Locus At3g48730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18049807 of line 362C09 Chr3:18049807
   
Insertion allele amplicon 8474 - AP22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP22: 5'-TGGAAGTGTTTGAAGAGCTTATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18049264
   
Insertion allele amplicon 8474 - BV84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV84: 5'-TCAAAACTGGAAAGAAACAAACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18050327
   
wt amplicon (over InsPos) AP22 - BV84
wt amplicon start Chr3:18049264
wt amplicon end Chr3:18050327
wt amplicon size 1064 bp
Locus specific primer BV84: 5'-TCAAAACTGGAAAGAAACAAACCT-3'
2nd locus specific primer AP22: 5'-TGGAAGTGTTTGAAGAGCTTATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11