Sequences from confirmation amplicon


Confirmation sequences for line 362D05 and hit At3g19590 are shown.
Line ID 362D05
Locus At3g19590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6807205
Primers used for PCR IL03 - 8474, show primer details
   
Sequence Name 13-K022406-022-362-D05C-8409
GenBank Accession LN491849 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K022406-022-362-D05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAAAGAGAAAAGAA
GAACACAAGGAATATACTTTTTAGCTTGAGCAGCCTCTGACAGATCAAAAAACTCCATTG
CAACCCTTCCTTCAACAGAGCTAAGAGCATAGCCTGTAAATTTTCAATTTCCGAAATTCA
TTGGTATGGAAGAAGAGGAACTGAGAGTATACTTTGGAATTGGAAGAAAAAATTAGCAAG
GACAGAAACAATATGTCAGTCATCTAACCTGTTCCATTAGGATAACAACGCACACATCTC
GTCTGGTATTTCAGTGAAGACTCCCTTCTTTGCTCAGGCTGAGACATATTTCTGAGATCA
TAGATGTTTACATGCCTTCCTGCTGTAGCCACTACCAAACGATGTCCAACAAGAGACATA
GAGTAAACACGTTCTGGTTGCAAATATGTTCCCACTTGGGTGCGTTCAGGCCCACTAGCG
CCTCTTGGATCCCAACATTTAACTGTTTTATC
   
Sequence Name 14-K022406-022-362-D05C-IL03
GenBank Accession LN492318 [GenBank]
Sequencing primer gene specific primer, IL03: 5'-CACTGGATCTTGGGATAAAACAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K022406-022-362-D05C-IL03
TGTTGGGATCCAAGAGGCGCTAGTGGGCCTGAACGCACCCAAGTGGGAACATATTTGCAA
CCAGAACGTGTTTACTCTATGTCTCTTGTTGGACATCGTTTGGTAGTGGCTACAGCAGGA
AGGCATGTAAACATCTATGATCTCAGAAATATGTCTCAGCCTGAGCAAAGAAGGGAGTCT
TCACTGAAATACCAGACGAGATGTGTGCGTTGTTATCCTAATGGAACAGGTTAGATGACT
GACATATTGTTTCTGTCCTTGCTAATTTTTTCTTCCAATTCCAAAGTATACTCTCAGTTC
CTCTTCTTCCATACCAATGAATTTCGGAAATTGAAAATTTACAGGCTATGCTCTTAGCTC
TGTTGAAGGAAGGGTTGCAATGGAGTTTTTTGATCTGTCAGAGGCTGCTCAAGCTAAAAA
GTATATTCCTTGTGTTCTTCTTTTCTCTTTTCCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11