Sequences from confirmation amplicon


Confirmation sequences for line 363D04 and hit At1g27940 are shown.
Line ID 363D04
Locus At1g27940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9734982
Primers used for PCR JG43 - 8474, show primer details
   
Sequence Name 02-K033877-022-363-D04C-8409
GenBank Accession LN486927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K033877-022-363-D04C-8409
AAGCCATTTACAATTGAATATATCCTGATAGTTTGAAGTCAATTGGACATGAAGTCAATT
GAATACTCATTGCTGCATTTACAGTCACTGCATTGGCACTTGCCTTTTTCTATAGTTGGC
GTGTTGCAGCTGTGGTAACGGCTTGTTTCCCTCTTCTCATTGCGGCTTCACTTACCGAGG
TAACTCAAAATAAAAAAAATCCTCTCAACCATATTTTGAATCTTGAAACTGAATAAGCTT
GAATATGACATTTTGCAGCAACTATTTTTAAAAGGCTTTGGAGGAGATTACACAAGAGCT
TACTCTAGGGCAACTTCAGTGGCACGTGAAGCGATTGCAAATATAAGAACTGTCGCTGCA
TATGGTGCCGAAAAGCAGATCTCGGAACAGTTTACTTGTGAGCTAAGCAAGCCCACCAAG
AATGCCTTTGTCAGAGGTCATATCTCTGGATTTGGATACGGTTTGTCTCAGTTTCTTGCG
TTCTGTTCCTACGCTCTTGGTCTCTGGTACGTATCGGTTTTGATCAATCATAAGGAGACG
AATTTTGGGGATAGTATCAAATCCTTCATGGTGTTGATCGTCACAGCTTTCTCAGTTTCG
GAAACGCTTGCTTTGACCCCAGATATCGTGAAGGGCACGCAAGCACTCGGGTCAGTTTTC
AGGGTTTTACATAGAGAGACCAAGATATC
   
Sequence Name 58-K033877-022-363-D04C-JG43
GenBank Accession LN509627 [GenBank]
Sequencing primer gene specific primer, JG43: 5'-CTGGTCTGGAGATATCTTGGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K033877-022-363-D04C-JG43
TGACCCGAGTGCTCTGCGTGCCCTTCACGATATCTGGGGGTCAAAGCAAGCGTTTCCGAA
ACTGAGAAAGCTGTGACGATCAACACCATGAAGGATTTGATACTATCCCCAAAATTCGTC
TCCTTATGATTGATCAAAACCGATACGTACCAGAGACCAAGAGCGTAGGAACAGAACGCA
AGAAACTGAGACAAACCGTATCCAAATCCAGAGATATGACCTCTGACAAAGGCATTCTTG
GTGGGCTTGCTTAGCTCACAAGTAAACTGTTCCGAGATCTGCTTTTCGGCACCATATGCA
GCGACAGTTCTTATATTTGCAATCGCTTCACGTGCCACTGAAGTTGCCCTAGAGTAAGCT
CTTGTGTAATCTCCTCCAAAGCCTTTTAAAAATAGTTGCTGCAAAATGTCATATTCAAGC
TTATTCAGTTTCAAGATTCAAAATATGGTTGAGAGGATTTTTTTTATTTTGAGTTACCTC
GGTAAGTGAAGCCGCAATGAGAAGAGGGAAACAAGCCGTTACCACAGCTGCAACACGCCA
ACTATAGAAAAAGGCAAGTGCCAATGCAGTGACTGTAAATGCAGCAATGAGTATTCAATT
GACTTCATGTCCAATTGACTTCAAACTATCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATC

Last Updated on 06.12.2018 16:11