Sequences from confirmation amplicon


Confirmation sequences for line 363E11 and hit At3g18460 are shown.
Line ID 363E11
Locus At3g18460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6333853
Primers used for PCR MG16 - 8474, show primer details
   
Sequence Name 71-K031692-022-363-E11C-8409
GenBank Accession LN512489 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K031692-022-363-E11C-8409
ATTTACAATTGAATATATCCTGGACATGAAGCCATTGCGTTTGGTTTTGGGAATGTACCT
GGTTGTCCCGGTTAACCGTTGGTTGAACTTGAACCGGAGGTGGTTGTGCTTCATTCGGCT
GACTACCTGGTCGACCCATATATATATAGTATTAATTTCCCGGCTAGATTTTTGCAACAC
AAAACGTTTGCTTCTTCGTTTGGTCTTTAAGTTTTTTGTTATAACGAATAGCTCTTATCG
TTTTTTTTGTGAAAGTTATGGATGAAGGTTAGGTTTATATAAACTAAGGTTTGAATTAGT
TTAAACTTTCTTGAGATGCTCTACTTTTCTTCGCATAGTTATTTCCATCTTGTATATAAT
TAACTAAATTCATTTGTATAGTTACTACTTTGAAACACTATTCCCCAAAA
   
Sequence Name 23-K031692-022-363-E11C-MG16
GenBank Accession LN496445 [GenBank]
Sequencing primer gene specific primer, MG16: 5'-TTTTTGGGGAATAGTGTTTCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K031692-022-363-E11C-MG16
TACAGATGGAAATACTATGCGAAGAAAAGTAGAGCATCTCAAGAAAGTTTAAACTAATTC
AAACCTTAGTTTATATAAACCTAACCTTCATCCATAACTTTCACAAAAAAAACGATAAGA
GCTATTCGTTATAACAAAAAACTTAAAGACCAAACGAAGAAGCAAACGTTTTGTGTTGCA
AAAATCTAGCCGGGAAATTAATACTATATATATATGGGTCGACCAGGTAGTCAGCCGAAT
GAAGCACAACCACCTCCGGTTCAAGTTCAACCAACGGTTAACCGGGACAACCAGGTACAT
TCCCAAAACCAAACGCAATGGCTTCATGTCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAAT
GGG

Last Updated on 06.12.2018 16:11