Sequences from confirmation amplicon


Confirmation sequences for line 363F05 and hit At3g24040 are shown.
Line ID 363F05
Locus At3g24040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8681928
Primers used for PCR GE20 - 8474, show primer details
   
Sequence Name 11-K033717-022-363-F05C-GE20
GenBank Accession LN491012 [GenBank]
Sequencing primer gene specific primer, GE20: 5'-TCTGTTTTCTGCATTTATCTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K033717-022-363-F05C-GE20
CATATTCAGTGTTGTGCAATCATTTGGATTGATATTATCATGGAAGATTACTTTTTCCTA
ATTATGCTGTTTTGTAGAACCTTAATCCCTTTTTCTTTTACATGGTTAGCAGTTTCTTGG
CACCATTTCATAATTTGGTAGGTAGATACGTGATAGTGTAGAAGTGGATAAGTTTAGGTG
AAAGCTCTATCAGAATATAGACTACATGACGTCTTGTTTATCAGTAATGCTATTAGCTGC
TTTCATTTGTGATCACATTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 91-K033717-022-363-F05C-8409
GenBank Accession LN515085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K033717-022-363-F05C-8409
TTACAATTGAATAATCCTGAAATGTGATCACAAATGAAAGCAGCTAATAGCATTACTGAT
AAACAAGACGTCATGTAGTCTATATTCTGATAGAGCTTTCACCTAAACTTATCCACTTCT
ACACTATCACGTATCTACCTACCAAATTATGAAATGGTGCCAAGAAACTGCTAACCATGT
AAAAGAAAAAGGGATTAAGTTTCTACAAAACAGCATAATTAGGAAAAAGTAATCTTCCAT
AATAATATCAATCCAAATGATTGCACAACACTGAATATGCTAAATAGCAAACAAGGTGGT
CAATTAGTAATGATCCACAGATAAATGCAGAAAACAG

Last Updated on 06.12.2018 16:11