Sequences from confirmation amplicon


Confirmation sequences for line 363G01 and hit At1g15040 are shown.
Line ID 363G01
Locus At1g15040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5180889
Primers used for PCR 06bn - 8474, show primer details
   
Sequence Name 49-K107839-0022-363-G01C-06bn
GenBank Accession LN506862 [GenBank]
Sequencing primer gene specific primer, 06bn: 5'-AAGTACGTGGACTTCGTAGGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K107839-0022-363-G01C-06bn
CGTCCCTGTCTTGTCCCCGTGTCAATGGGATCCATTCTATGCTCCAGAGCTTCGAGCCTA
TTCATGGTGTCCTCCTCTGCGAAGGAGAAGATGTCGATCCTTCTCTCTACGCGGACGATG
AATTATCAGACCTCTCGCCAGAAGACATGGAGGAGATTAAGAAGGCGCATGCAGAGGACA
TGACCATCGACCGAGAGAAAGACAGCATTGAGCTGACTCTAGCTAGACTCTGTCTCGAAA
GAAACATTCCTTTCTTAGGCATTTGTCGTGGCTCTCAGATTCTCAACGTTGTCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTA
   
Sequence Name 81-K107840-0022-363-G01C-8409
GenBank Accession LN514079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K107840-0022-363-G01C-8409
ATTTACAATTGAATAATCCTGACAACGTTGAGAATCTGAGAGCCACGACAAATGCCTAAG
AAAGGAATGTTTCTTTCGAGACAGAGTCTAGCTAGAGTCAGCTCAATGCTGTCTTTCTCT
CGGTCGATGGTCATGTCCTCTGCATGCGCCTTCTTAATCTCCTCCATGTCTTCTGGCGAG
AGGTCTGATAATTCATCGTCCGCGTAGAGAGAAGGATCGACATCTTCTCCTTCGCAGAGG
AGGACACCATGAATAGGCTCGAAGCTCTGGAGCATAGAATGGATCCCATTGACACGTGGG
ACAATGACAGGGACGGCACCGGAGGAGACGATGAGATCGAGATGGTATTCTCCTACGAAG
TCCACGTACT

Last Updated on 06.12.2018 16:11