Sequences from confirmation amplicon


Confirmation sequences for line 363H05 and hit At1g60080 are shown.
Line ID 363H05
Locus At1g60080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22154315
Primers used for PCR GE19 - 8474, show primer details
   
Sequence Name 03-K033703-022-363-H05C-8409
GenBank Accession LN487376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K033703-022-363-H05C-8409
ATTTACAATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTGGAGCTTGCATGT
TGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAATTTCCATTGAGAACTGAGCCATG
TGCACCTTCCCCCCAACACGGTGAGCGACGGGGCAACGGAGTGATCCACATGGGACTTTT
AAACATCATCCGTCGGATGGCGTTGCGAGAGAAGCAGTCGATCCGTGAGATCAGCCGACG
CACCGGGCAGGCGCGCAACACTTGAGCTGCATCTGGTATTCCCATGTTTTCTTTAGCTCT
GCTTCATGTCACAAAGATAAAGAGAATCATAAACCAAAAGTCTCTGAGTCTGTGCTTCAT
CAACACAGTCTCAAGGAATCACAAAGCTTACCTTGGGACAAAAGAAAGAACTGCCTTTGG
TACACGAAACTGTTGTAGTGAAGAGGAAACACAAGCAATAGAGTGTAAGAAGAAGATTGA
GAGCTCGCGGTGTCGTAAGTAATGGGGATTTCGACGGAGACGGTGACGGTGACGACGGCT
AGGTTTATCTGAGTTTTTAACTAAAGTACTTCTTCATTCTAAATGGGCCCCAAACAAAGC
CCAATAATGTTGCGAAGCCCAAAGAAATGCGAAATAAGAGATTTGTTTGCTTCACAAGAC
ATGAGTATATTGAAATTTGGGTTTTCAATTAGATGAATTTGGCGAAAATATGAGGCGCAC
TTGATTATGAGCTG
   
Sequence Name 03-K033703-022-363-H05C-GE19
GenBank Accession LN487377 [GenBank]
Sequencing primer gene specific primer, GE19: 5'-TTTTCCCAAATTCAGCTCATAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K033703-022-363-H05C-GE19
CAATTCTCTAATTGAAACCCAAATTTCAATATACTCATGTCTTGTGAAGCAAACAAATCT
CTTATTTCGCATTTCTTTGGGCTTCGCAACATTATTGGGCTTTGTTTGGGGCCCATTTAG
AATGAAGAAGTACTTTAGTTAAAAACTCAGATAAACCTAGCCGTCGTCACCGTCACCGTC
TCCGTCGAAATCCCCATTACTTACGACACCGCGAGCTCTCAATCTTCTTCTTACACTCTA
TTGCTTGTGTTTCCTCTTCACTACAACAGTTTCGTGTACCAAAGGCAGTTCTTTCTTTTG
TCCCAAGGTAAGCTTTGTGATTCCTTGAGACTGTGTTGATGAAGCACAGACTCAGAGACT
TTTGGTTTATGATTCTCTTTATCTTTGTGACATGAAGCAGAGCTAAAGAAAACATGGGAA
TACCAGATGCAGCTCAAGTGTTGCGCGCCTGCCCGGTGCGTCGGCTGATCTCACGGATCG
ACTGCTTCTCTCGCAACGCCATCCGACGGATGATGTTTAAAAGTCCCATGTGGATCACTC
CGTTGCCCCGTCGCTCACCGTGTTGGGGGGAAGGTGCACATGGCTCAGTTCTCAATGGAA
ATTATCTGCCTAACCGGCTCAGTTCTGCGTAGAAACCAACATGCAAGCTCCACCGGGTGC
AAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAGATCTTTATTTCGACGTGTC

Last Updated on 06.12.2018 16:11