Sequences from confirmation amplicon


Confirmation sequences for line 364A11 and hit At2g45950 are shown.
Line ID 364A11
Locus At2g45950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18904743
Primers used for PCR BW51 - 8474, show primer details
   
Sequence Name 48-K106149-0022-364-A11C-BW51
GenBank Accession LN506510 [GenBank]
Sequencing primer gene specific primer, BW51: 5'-AACGTAGAACGTGGGTTTACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K106149-0022-364-A11C-BW51
AACATCAAATCAAACTGTTACGTGAGGACACGCATCAAACGCCGGTGGTGACTGACTGGT
GAACCATTCTCTTTTCTTTCTATTTGCTCACATACTTAACCAAAACTGTTCCATTATCAG
CTTCAATCGTGTCATGGAGATTGACCTTCAAATTCAAACCACAAGCCCCATCACATAGTC
ATCTAGAGTATGGCTAAAAAAGAGTAAACATACATGGCCGCCAAATAGATACCATACCTG
TATGCCGTCTTGTAGTCCCGTTGCAATGAGTAGTTCCCAGATAAGGGAATTAGGGTTCTT
ATAGGGTTTCGCTCATGTGTTGAGCAATGAGTATATTCAATTGCGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTA
   
Sequence Name 64-K106150-0022-364-A11C-8409
GenBank Accession LN511208 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K106150-0022-364-A11C-8409
CGGACTGAGCCTTTAAATTGAATAATCCGCAATTGAATATACTCATTGCTCAACACATGA
GCGAAACCCTATAAGAACCCTAATTCCCTTATCTGGGAACTACTCATTGCAACGGGACTA
CAAGACGGCATACAGGTATGGTATCTATTTGGCGGCCATGTATGTTTACTCTTTTTTAGC
CATACTCTAGATGACTATGTGATGGGGCTTGTGGTTTGAATTTGAAGGTCAATCTCCATG
ACACGATTGAAGCTGATAATGGAACAGTTTTGGTTAAGTATGTGAGCAAATAGAAAGAAA
AGAGAATGGTTCACCAGTCAGTCACCACCGGCGTTTGATGCGTGTCCTCACGTAACAGTT
TTGATTTTGATTGTTTTACTCTCTAGTGGTCCATCTTTTTCTTTGTAAACCCACGTTCTA
CGT

Last Updated on 06.12.2018 16:11