Sequences from confirmation amplicon


Confirmation sequences for line 364F11 and hit At2g02020 are shown.
Line ID 364F11
Locus At2g02020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:479655
Primers used for PCR BW53 - 8474, show primer details
   
Sequence Name 70-K106152-0022-364-F11C-8409
GenBank Accession LN512393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K106152-0022-364-F11C-8409
CGGACATGAGCCATTATTTCCCGTAAGCATTTTCTTGAAAATAACTTTGCTTTATGTTCT
GTTCTTGGCTTGATGTTCTGTTCTTTGATGTTGTTTTAAGGGAATGGTTGCATTGACACT
CTCAGCTTCAGTTCCGGGTCTTAAGCCAGCGGAATGCATTGGCTCTCTATGTCCACCAGC
AACAATGGTTCAGTCTACGGTTTTATTTTCAGGGCTTTACCTTATCGCTCTTGGCACTGG
AGGAATCAAACCATGTGTCTCATCCTTTGGTGCTGATCAGTTTGATAAGACCGATCCAAG
CGAACGAGTCAGAAAAGCTTCTTTCTTTAACTGGTTTTACTTCACTATCAACATTGGTGC
TTTTGTTTCATCTACTGTTCTAGTTTGGATTCAAGAGAATTATGGATGGGAATTAGGATT
CTTGATACCTACCGTGTTCATGGGACTTGCTACTATGAGTTTCTTCTTTGGCACGCCGCT
TTATAGATTTCAGAAACCGAGAGGTAGCCCGATTACTAGCGTCTGCCAAGTTCTTGTAGC
CGCATACCGTAAATCGAATCTCAAGGTCCCTGAAGACTCCACGGACGGTTACAAGAAAAT
CTTTTTCCCTCTCATTTCTTGTTTGAATTGAGCATTAGTCTATTAACGGTTAAATAAACG
GGTTGTGATATCAATGTACAGGTTTCTTGACAAAGCGGCAATTATATCAGAAGGAGATGC
AAACACTAACCCGTGGAAGCTATGTACCGTGATAAAGGTCGAAG
   
Sequence Name 66-K106151-0022-364-F11C-BW53
GenBank Accession LN511614 [GenBank]
Sequencing primer gene specific primer, BW53: 5'-CTTCGACTTGAGTCACGGTACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106151-0022-364-F11C-BW53
TTCTGATTATTGCCGCTTTGTCAGAAACCTGTACATTGATATCACAACCCGTTTATTTAA
CCGTTAATAGACTAATGCTCAATTCAAACAAGAAATGAGAGGGAAAAAGATTTTCTTGTA
ACCGTCCGTGGAGTCTTCAGGGACCTTGAGATTCGATTTACGGTATGCGGCTACAAGAAC
TTGGCAGACGCTAGTAATCGGGCTACCTCTCGGTTTCTGAAATCTATAAAGCGGCGTGCC
AAAGAAGAAACTCATAGTAGCAAGTCCCATGAACACGGTAGGTATCAAGAATCCTAATTC
CCATCCATAATTCTCTTGAATCCAAACTAGAACAGTAGATGAAACAAAAGCACCAATGTT
GATAGTGAAGTAAAACCAGTTAAAGAAAGAAGCTTTTCTGACTCGTTCGCTTGGATCGGT
CTTATCAAACTGATCAGCACCAAAGGATGAGACACATGGTTTGATTCCTCCAGTGCCAAG
AGCGATAAGGTAAAGCCCTGAAAATAAAACCGTAGACTGAACCATTGTTGCTGGTGGACA
TAGAGAGCCAATGCATTCCGCTGGCTTAAGACCCGGAACTGAAGCTGAGAGTGTCAATGC
AACCATTCCCTTAAAACAACATCAAAGAACAGAACATCAAGCCAAGAACAGAACATAAAG
CAAAGTTATTTTTCAAGAAAATGCTTACGGTGAAATAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11