Sequences from confirmation amplicon


Confirmation sequences for line 365A04 and hit At1g68370 are shown.
Line ID 365A04
Locus At1g68370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25633032
Primers used for PCR 38rn - 8474, show primer details
   
Sequence Name 12-K116188-0022-365-A04C-38rn
GenBank Accession LN491735 [GenBank]
Sequencing primer gene specific primer, 38rn: 5'-ACAGAGTGCTTCAATCGTGTAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116188-0022-365-A04C-38rn
CTAAGGTCTCAAAAATGCTCCAGCTATCGTAAGTAAAGTAATAAGTCATACCATAAACTG
CAAATATATGCGTGCCAGCTTTCAGTTCCGAAACCTCACAAGGTTGAAGACCTTCCAATC
GCTTGAAGAAAGCAGATTCAGGGTCCTTGGCTGCAGCCAACTGCACAATAACCACAATGT
GGATTGCTTTAGTACCTCTGCATATTCTAATATGCCAGCTAATTATGAAAGGAAAAGAGC
TCAAAACTTGCATACCGCATTGACAGTTGTATCCTGAATTATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K116187-0022-365-A04C-8409
GenBank Accession LN487183 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116187-0022-365-A04C-8409
TATAATTTCAGGATACACTGTCAATGCGGTATGCAAGTTTTGAGCTCTTTTCCTTTCATA
ATTAGCTGGCATATTAGAATATGCAGAGGTACTAAAGCAATCCACATTGTGGTTATTGTG
CAGTTGGCTGCAGCCAAGGACCCTGAATCTGCTTTCTTCAAGCGATTGGAAGGTCTTCAA
CCTTGTGAGGTTTCGGAACTGAAAGCTGGCACGCATATATTTGCAGTTTATGGTATGACT
TATTACTTTACTTACGATAGCTGGAGCATTTTTGAGACCTTAGTTTAACTGGGCTCTAGT
TACTTTTCAGGTGATAATTTCTTTAAGACTGCATCTTACACGATGAGGCAACTCT

Last Updated on 06.12.2018 16:11