Sequences from confirmation amplicon


Confirmation sequences for line 365A07 and hit At1g12010 are shown.
Line ID 365A07
Locus At1g12010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4057153
Primers used for PCR BW55 - 8474, show primer details
   
Sequence Name 05-K106136-0022-365-A07C-8409
GenBank Accession LN488361 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106136-0022-365-A07C-8409
TTACATTGAAAAGAGCGGAAACGACAATTACCGCCAATCACCATCTTTAAGAAGCTGGAG
ACCACTGACCTTATCATCTTGAAACAGCAAAATGAGGCCTCCTGCATCTGTGTGAGCCCT
AAGCCCTTTGATCATCTCTGGTTTAGGACATGGTGGATAGTTGCTAAGCTTTGTCGCAAA
AGTTGGACCCGTTGTCCCATGAAACACCTTCTTCAAGTACCCTTTCTCCAACCCTAGATT
CTCACACAACAAGTCCAATAGCTCTTCAGCTAGAATCTCAAGCCTCTTCCCAAAATCCTT
CATTGCCAATCTGTTAAGAGACATGGAAAGCAAAGTAATCAATAATTTTGGAAGATTGTG
AGGCGTTTTCAAAGCTGGATCAAAGNCATGTTTG
   
Sequence Name 05-K106135-0022-365-A07C-BW55
GenBank Accession LN488360 [GenBank]
Sequencing primer gene specific primer, BW55: 5'-ACAAACAGACTTTGATCCAGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106135-0022-365-A07C-BW55
TCAATTTTGATACTTTGCTTTCCTGTCTCTTAACAGATTGGCAATGAAGGATTTTGGGAA
GAGGCTTGAGATTCTAGCTGAAGAGCTATTGGACTTGTTGTGTGAGAATCTAGGGTTGGA
GAAAGGGTACTTGAAGAAGGTGTTTCATGGGACAACGGGTCCAACTTTTGCGACAAAGCT
TAGCAACTATCCACCATGTCCTAAACCAGAGATGATCAAAGGGCTTAGGGCTCACACAGA
TGCAGGAGGCCTCATTTTGCTGTTTCAAGATGATAAGGTCAGTGGTCTCCAGCTTCTTAA
AGATGGTGATTGGCGGTAATTGTCGTTTCCGCTCTTTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11