Sequences from confirmation amplicon


Confirmation sequences for line 365B11 and hit At4g02020 are shown.
Line ID 365B11
Locus At4g02020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:890896
Primers used for PCR 23cs - 8474, show primer details
   
Sequence Name 27-K109134-0022-365-B11C-23cs
GenBank Accession LN498425 [GenBank]
Sequencing primer gene specific primer, 23cs: 5'-TCTAAATGGAACTTTTGAGTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K109134-0022-365-B11C-23cs
AAGATATTACACCGATCATGTGGTTTTTTCATACAAAATGGATATGAGAGAGAAGCATTT
AGTACCTTAGCGTAGCAATTGGGTTTAGCTGAGTGATTGGCAAATTTCAGCTTGTCACCT
TTGCGTTGAGCATCGAGGACGTACTGCAAATATCAAAAGCTTACTGACATATGCGAAATT
CAAGAGGAAACTATCAGAAAGAAGGGTGAGAGATTTGGTCACCTGATCATTCAAGTCAAA
GAGGAAGGACGAATTTGCCCGGTCATATATTTTCCCACGCTTATCCGCCTCATGGTGTGA
GATCAATTCACCGGTGTATTCTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 11-K109133-0022-365-B11C-8409
GenBank Accession LN491153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109133-0022-365-B11C-8409
ATTTCATTGAATTATCCTGGAGAATACACCGGTGAATTGATCTCACACCATGAGGCGGAT
AAGCGTGGGAAAATATATGACCGGGCAAATTCGTCCTTCCTCTTTGACTTGAATGATCAG
GTGACCAAATCTCTCACCCTTCTTTCTGATAGTTTCCTCTTGAATTTCGCATATGTCAGT
AAGCTTTTGATATTTGCAGTACGTCCTCGATGCTCAACGCAAAGGTGACAAGCTGAAATT
TGCCAATCACTCAGCTAAACCCAATTGCTACGCTAAGGTACTAAATGCTTCTCTCTCATA
TCCATTTTGGTATGAAAAAACCACATGATCGGTTAGTAATTATACCTTCTTTCTCACCTC
TAGCCACTCAAAAGTTCCATTTAGA

Last Updated on 06.12.2018 16:11