Genotyping and primer details


Genotyping and primer details for 365B11 - At4g02020
Line ID 365B11
Locus At4g02020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:890896 of line 365B11 Chr4:890896
   
Insertion allele amplicon 8474 - 23cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23cs: 5'-TCTAAATGGAACTTTTGAGTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:891263
   
wt amplicon (over InsPos) 02ti - 23cs
wt amplicon start Chr4:890339
wt amplicon end Chr4:891263
wt amplicon size 925 bp
Locus specific primer 23cs: 5'-TCTAAATGGAACTTTTGAGTGGCT-3'
2nd locus specific primer 02ti: 5'-GGTGCTCAAAAAGCTGTAAAAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11