Sequences from confirmation amplicon


Confirmation sequences for line 365C01 and hit At2g44450 are shown.
Line ID 365C01
Locus At2g44450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18343625
Primers used for PCR NH05 - 8474, show primer details
   
Sequence Name 30-K114963-0022-365-C01C-NH05
GenBank Accession LN499843 [GenBank]
Sequencing primer gene specific primer, NH05: 5'-TGAAAACCATGAGATTCTTCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114963-0022-365-C01C-NH05
TGATAATGCTGAAGTTGTAGATGTTACATACTCTTTGCCTATATCGTTTTCACATTCTAT
TTTTTTTTTAACTTTTTCAACAAGTTTAATATTAAGTTTCCTTATAAAACGTACGTAATT
ACATCGAAAGATGATTTCCATCAGCTATACATCAATCGGGTTTCGTTGTGAAAATAGCTA
AAACCATTTCTCATTATTTCCAAAGGGGGGAAAAAATATATAAATCTCTCAAGAGTAACA
GAGAAACTAATATAATGAAATATACATATCTCATCATCATCAATCGTTTTTCTTTTCATT
CAATAACTTTCTGAACCATTCAGCTGATTTCTTGGGATATCTCTAACATCCGTCTTTGAA
ATCCACATAAACCAGTCCAAACCGGACCGTGTATCCCATTGCCCATTCGAAATTGTCTAG
CAACGAAAATAAATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCA
   
Sequence Name 14-K114962-0022-365-C01C-8409
GenBank Accession LN492628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114962-0022-365-C01C-8409
GGCATGGGATACACGGTCCGGTTTGGACTGGTTTATGTGGATTTCAAGACGGATGTAAGA
GATATCCCAAGAAATCAGCTGAATGGTTCAGAAAGTTATTGAATGAAAAGAAAAACGATT
GATGATGATGAGATATGTATATTTCATTATATTAGTTTCTCTGTTACTCTTGAGAGATTT
ATATATTTTTTCCCCCCTTTGGAAATAATGAGAAATGGTTTTAGCTATTTTCACAACGAA
ACCCGATTGATGTATAGCTGATGGAAATCATCTTTCGATGTAATTACGTACGTTTTATAA
GGAAACTTAATATTAAACTTGTTGAAAAAGTTAAAAAAAAAATAGAAGGTGAAAACGATT
TAGGCAAAGAGTATGTAACATCTACAACTTTCAGCATATATCATTTTGAAAAAAAATGTT
CAATCTTTAAATTTTTGAAGAATCTCAGGGGTTTTC

Last Updated on 06.12.2018 16:11