Sequences from confirmation amplicon


Confirmation sequences for line 365C01 and hit At3g14100 are shown.
Line ID 365C01
Locus At3g14100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4675659
Primers used for PCR BW56 - 8474, show primer details
   
Sequence Name 03-K106136-0022-365-C01C-8409
GenBank Accession LN487497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K106136-0022-365-C01C-8409
TTTACATTGAATATATCCTGACATAATCGACCATGTAGATTTCCCGGACATGATAACAAA
TGTCAGATTGCATGCTAAGTTTCAGATGAAGTAACTTTTGGGAGCAGTAAGATCAAATAG
AGAGGGTTTAGAGTGGTAAGCCAAACTTAAAAGTACCTTTATCTGTCTGTTAAAGAGGTA
AGGCTGAGTGTTACCCATCTGAATAGCAAGAGCAGCTTCGGGATGAGTGTTATATCTCAC
GAAACCAAAGCCTTTGTCTCGTTGGACACGGACCTCCTCAATAACTCCAGCGCCAAGAGC
ATGGAAGTAACGGTGTAGATCAAGCTGAGTTACCTGCAAGATAGATAGCTGTAACGATGT
ACCCGAAATCTACTAATGTAAAACTTCATCAAAGAACCTCAAATAAACTGTTTTTGTTGT
GATAGTATTACAAGGAGCACTTCCTAGTGGATAATGTACCTCTGGAGCAAGGTTTCCCAC
ATAAACAGTGGTAAACTGAGAATTATTTTCAGGTGTTTCCTCATTTAATGTCTCTTTACC
ATCCTCTGCACAATCAAACGACCAATATGGCTTTGTTAACTTAAAGTTTTTCATATTACT
CAGGGAAAATCAATGTAGGTTCGT
   
Sequence Name 03-K106135-0022-365-C01C-BW56
GenBank Accession LN487496 [GenBank]
Sequencing primer gene specific primer, BW56: 5'-CCTCAAATGGAATTTACGAACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K106135-0022-365-C01C-BW56
GAAACTTTAAGTTACAAAGCCATATTGGTCGTTTGATTGTGCAGAGGATGGTAAAGAGAC
ATTAAATGAGGAAACACCTGAAAATAATTCTCAGTTTACCACTGTTTATGTGGGAAACCT
TGCTCCAGAGGTACATTATCCACTAGGAAGTGCTCCTTGTAATACTATCACAACAAAAAC
AGTTTATTTGAGGTTCTTTGATGAAGTTTTACATTAGTAGATTTCGGGTACATCGTTACA
GCTATCTATCTTGCAGGTAACTCAGCTTGATCTACACCGTTACTTCCATGCTCTTGGCGC
TGGAGTTATTGAGGAGGTCCGTGTCCAACGAGACAAAGGCTTTGGTTTCGTGAGATATAA
CACTCATCCCGAAGCTGCTCTTGCTATTCAGATGGGTAACACTCAGCCTTACCTCTTTAA
CAGACAGATAAAGGTACTTTTAAGTTTGGCTTACCACTCTAAACCCTCTCTATTTGATCT
TACTGCTCCCAAAAGTTACTTCATCTGAAACTTAGCATGCAATCTGACATTTGTTATCAT
GTCCGGGAAATCTACATGGTCGATTATGTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11