Sequences from confirmation amplicon


Confirmation sequences for line 365C06 and hit At1g62320 are shown.
Line ID 365C06
Locus At1g62320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23044022
Primers used for PCR 01mm - 8474, show primer details
   
Sequence Name 76-K114351-0022-365-C06C-01mm
GenBank Accession LN513442 [GenBank]
Sequencing primer gene specific primer, 01mm: 5'-TAAGATGCCAGAGCCTGAACTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K114351-0022-365-C06C-01mm
GTAATCAAAAAATGTTTTGTTCTTGCATACAGTTACTAATTATAGCTTTGTTTTTTTTTC
CAAAGGGTTTTTTTTTTGTTTACCAATTTAAAGGCCTAAAAACCTTGGGCCCAATGGCTT
ACTTTCCGGGGCAATTTTAATCCCCGGAAATTGGAACAATGAAGGATTGCCCCCTCCCAA
ACCTCCTAAGGGAACCCCCAATAAAATCCAAAACCCTTCCATTTCCAAAGGCCAAAGGGG
AACAAAACGGTTTTTTAATGAAAAAAAAATAATTAATTCCCCCCTCCGAATTTTGGTAAA
AACCTTAAAAAGAATAAAATTTTTCCTAATTTGGGAAAAAGGGTTTGGGCCCCTCCTGGG
AAGGCCTAAGCCTTCCCCTTTTGGACTTGCCTTTTCCCAAAGAAGGAAATTAAAAAAATT
TCCGCCAAGGGGTTTTTTTTTCCCCCAACCAAAAAACCACCGGGCGAACAATTCCCCGGA
AAAAATAATGTTTTCCTTAAAAAAAAAACCCCCCTTGCCTCCAAAATCCATTTGAAAAGG
CCTCCTGTCCCGGAAATCCCCCGGGAACCCCCATGGATTTGAAGGGCCAATTGGAAAAAA
AAAAAAAATAATTCCCCATTTTTTTTCCATTCCAAAA
   
Sequence Name 28-K114350-0022-365-C06C-8409
GenBank Accession LN498951 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114350-0022-365-C06C-8409
ATTGAAGCAAAGAGAGATTCTATATTAATGAAAACAATAATCTTTACAGTGAATTGATCA
GCACGTCGCTTCTCTGATTGGAGAAAAGATAACCGCATTGCAGCAATTTTCTCATATTCC
TTCATTAGTACATAGCAAGTCCAAAATGTGAATGCATAAGCCATCACAAGATGTGCCCAA
AACCTTTTTACCAAATTAAGAAAAATTCTAATCATTATAAAGTTTTTAACAAAAATCAGA
AGTAGCAACTACATAATTTTCTTTCACTAACAAACCTGTCTGATCCACGTTCGACATTCG
AAATCGAAAGCTTATCGATATTACTCGATGTTACATTACGAAGTTTTGCTAGCTGCAATC
CATCACTTGTCCAATTTACAGGTACTAAAATTGACCATGAAAGTAATGCAATCGGACCAA
AGATCTTAAGCCTATAAAATAGTAAACAAAAAAAAAACCCTTTAAAAAAAAAAAAAAAGC
TTTTATTTATAACTGGTTGGCAAAAAAAAAACTTTTTTGAATACCCCATCCAGTTAATCC
CCCAGTAAACCGCCAAATCCCAACCCGCCGGAATAAAAATTTTC

Last Updated on 06.12.2018 16:11