Sequences from confirmation amplicon


Confirmation sequences for line 365F01 and hit At5g48110 are shown.
Line ID 365F01
Locus At5g48110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19501695
Primers used for PCR 25qv - 8474, show primer details
   
Sequence Name 71-K115779-0022-365-F01C-25qv
GenBank Accession LN512612 [GenBank]
Sequencing primer gene specific primer, 25qv: 5'-TCATATAGCAGTTGATTCCTGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K115779-0022-365-F01C-25qv
CATTTCTACCAGATTTTGTAAGTTATATAAACGAAAGAATAGGTATATGTGATTCGTGTG
CATTCCAAATTGTACCTTGTAGGTGGCAACATCATCTCTGAGACGTGATTTTAGATCTAT
AGTTTGAATGAATTTCGGTCTTGATCTTATCCAGTCAAAAGCTTCTTTTCCTGCTTCCCC
CATACCAATGAAGGCGAACGCAACCGTGACATCTACTCCCCCAGTCACTATTCCAACCTC
CATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATAATAAT
   
Sequence Name 23-K115778-0022-365-F01C-8409
GenBank Accession LN496691 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K115778-0022-365-F01C-8409
GCATTTACTGGAGGTTGGAATAGTGACTGGGGGAGTAGATGTCACGGTTGCGTTCGCCTT
CATTGGTATGGGGGAAGCAGGAAAAGAAGCTTTTGACTGGATAAGATCAAGACCGAAATT
CATTCAAACTATAGATCTAAAATCACGTCTCAGAGATGATGTTGCCACCTACAAGGTACA
ATTTGGAATGCACACGAATCACATATACCTATTCTTTCGTTTATATAACTTACAAAATCT
GGTATGAAATGATAATTAGGATGAAATGGCTAGAGGAGAGATTGCGACAGGAATCAACTG
CTATATGA

Last Updated on 06.12.2018 16:11