Sequences from confirmation amplicon


Confirmation sequences for line 365G09 and hit At5g21150 are shown.
Line ID 365G09
Locus At5g21150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7194560
Primers used for PCR IF16 - 8474, show primer details
   
Sequence Name 10-K032413-022-365-G09C-IF16
GenBank Accession LN490539 [GenBank]
Sequencing primer gene specific primer, IF16: 5'-TAATTGAGCACTGTGATCTCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K032413-022-365-G09C-IF16
ATCTATCAATCAAAGACCAATATCTTAGAATAAACTCAAAAGCATATAAACTTGTGATCA
AAGAAATTTGACATACAGTTGATCTTTGCAGCTGTGTTCACTTAGTCCACTTATCTTGTA
TTCTCTATTGGAAAGAGTAATTTGAACTCTCAGATTCTTGAGGACACGTCGAGCCTGATG
AGATAAAATCAGATAAAATACATCAGTGAAATAGTACAACATAAAAGAAAATGATGTACC
TTGTTCCAGTCCATTCCGTATGGATCTTTCTTGTTCTGGTTAGCAAGCAGGAAATCAACT
ACAGGTCCAGGTTGTACTATCATCGTAGTTGAAGTGTCTGAAGAAATGCCAAAGCAAAAA
AATCAAAGTTTAGCAGCCGAATGTCAAGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTT
   
Sequence Name 10-K032413-022-365-G09C-8409
GenBank Accession LN490538 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K032413-022-365-G09C-8409
TACTTTGATTTTTTTGCTTTGGCATTTCTTCGACACTTCAACTACGATGATAGTACAACC
TGGACCTGTAGTTGATTTCCTGCTTGCTAACCAGAACAAGAAAGATCCATACGGAATGGA
CTGGAACAAGGTACATCATTTTCTTTTATGTTGTACTATTTCACTGATGTATTTTATCTG
ATTTTATCTCATCAGGCTCGACGTGTCCTCAAGAATCTGAGAGTTCAAATTACTCTTTCC
AATAGAGAATACAAGATAAGTGGACTAAGTGAACACAGCTGCAAAGATCAACTGTATGTC
AAATTTCTTTGATCACAAGTTTATATGCTTTTGAGTTTATTCTAAGATATTGGTCTTTGA
TTGATAGATTTACATGGAGGAAACCTAACGACAAGGGAGAATTTGAGGAGGTTGAGATCA
CATGCCTTCAATT
Primers used for PCR FQ21 - 8474, show primer details
   
Sequence Name 25-K035335-022-365-G09C-8409
GenBank Accession LN497346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K035335-022-365-G09C-8409
GCCATTTACAATTGAATCTAATACACATTAGATCTGGAAAAGAATTACCAATATTCAGGG
ATAAGCCTCCCTGAGTAGTTCTGAAACTTGAATGGAACCCTCTGCAACCACTGACACCTC
CACCAATAGGTACAAAGTTCTTTACGTCATTGTGGAAAAAGGACTGGCGAACAAGGAGGC
AACCTCTGCGAACAGATATGAGAACATGACATTAAAGATCAAGCAGAGAATTTATTTGAA
ATACAAATACCCAGAATATTTAACTTACTGCCTAGCTGCACTCTGGCGCAAAATAATATC
CAGCACTCTCAGAGCGTCTTGAAGATTCTCTGTCTCCTTCCCTTGAAGAGCGCTTGCAAT
AGCCTGCATGGGGATCTTTGCAGCATAACTTATCTCAACCATGAATTTCTTAGTTTGGTT
GGGACGCCTTGATCTTTTTCTATCAGCATCATTTGTATCATTTCCAGCGTGATTTCTGCA
GATAAAGAGGATATCAAAGCATATTAGACTTTGAAGAAAAGGAAACAGTCGAGAAAT
   
Sequence Name 25-K035335-022-365-G09C-FQ21
GenBank Accession LN497347 [GenBank]
Sequencing primer gene specific primer, FQ21: 5'-TGAAATTTCTCGACTGTTTCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K035335-022-365-G09C-FQ21
GCTTTGATATCCTCTTTATCTGCAGAAATCACGCTGGAAATGATACAAATGATGCTGATA
GAAAAAGATCAAGGCGTCCCAACCAAACTAAGAAATTCATGGTTGAGATAAGTTATGCTG
CAAAGATCCCCATGCAGGCTATTGCAAGCGCTCTTCAAGGGAAGGAGACAGAGAATCTTC
AAGACGCTCTGAGAGTGCTGGATATTATTTTGCGCCAGAGTGCAGCTAGGCAGTAAGTTA
AATATTCTGGGTATTTGTATTTCAAATAAATTCTCTGCTTGATCTTTAATGTCATGTTCT
CATATCTGTTCGCAGAGGTTGCCTCCTTGTTCGCCAGTCCTTTTTCCACAATGACGTAAA
GAACTTTGTACCTATTGGTGGAGGTGTCAGTGGTTGCAGAGGGTTCCATTCAAGTTTCAG
AACTACTCAGGGAGGCTTATCCCTGAATATTGGTAATTCTTTTCCAGATCTAATGTGTAT
TAGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCACGTCCCAATGG

Last Updated on 06.12.2018 16:11