Sequences from confirmation amplicon


Confirmation sequences for line 366B09 and hit At1g78320 are shown.
Line ID 366B09
Locus At1g78320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29468124
Primers used for PCR GE16 - 8474, show primer details
   
Sequence Name 06-K033703-022-366-B09C-8409
GenBank Accession LN488700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K033703-022-366-B09C-8409
ATTTACAATTGAATAATCCTGACACAAACCCGATTCTTCCTTCAGATCCTTACCAGAGAG
CTCAAGCTAGATTTTGGGCAGATTACATCGACAAGAAGGTTACATTTTTTCTTTTACGTT
ATTGAGTTCTTTGCACTATATAATTCCCTAACTATATAAGCAAAAAACGAATGATGTATT
GCTAATTGTTTTTTTTGGACAAACTGTGATCAGACATACGTGCCATGCAAGGCATTATGG
TCAGAATCAGGGGAGAAACAAGAGGCAGCGAAGATAGAGTTTATAGAAGTACTCAAGACA
CTTGATTCAGAGCTTGGAGACAAATATTATTTTGGAGGAAACGAATTTGGACTCGTTGAC
ATTGCCTTTATCGGATTCTATAGTTGGTTTCGTACGTACGAGGAGGTAGCGAATCTCAGC
ATCGTGTTAGAGTTTCCTAAGCTAATGGCGTGGGCACAGAGGTGTTTGAAGAGAGAGAGT
GTGGCTAAGGCCTTGCCTGACTCTGATAAGGTTCTTAAGTCAGTCTCTGATCATCGGAAG
ATTATTTTAGGAATCGATTAAGTTCACTTACCCCTGTTCTGTTTTCAATGTTT
   
Sequence Name 06-K033703-022-366-B09C-GE16
GenBank Accession LN488701 [GenBank]
Sequencing primer gene specific primer, GE16: 5'-AAAAACATGAAAAACAGAACAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K033703-022-366-B09C-GE16
TCTTCCGATGATCAGAGACTGACTTAAGAACCTTATCAGAGTCACCGCAAGGACTTAGCC
ACACTCTCTCTCTTCAAACACCTCTGCCCCCACAACCATTAGCTTAGGAAACTCTAACAC
GATGCTGAGATTCGCTACCTCCTCGTACGTACGAAACCAACTATAGAATCCGATAAAGGC
AATGTCAACGAGTCCAAATTCGTTTCCTCCAAAATAATATTTGTCTCCAAGCTCTGAATC
AAGTGTCTTGAGTACTTCTATAAACTCTATCTTCGCTGCCTCTTGTTTCTCCCCTGATTC
TGACCATAATGCCTTGCATGGCACGTATGTCTGATCACAGTTTGTCCAAAAAAAACAATT
AGCAATACATCATTCGTTTTTTGCTTATATAGTTAGGGAATTATATAGTGCAAAGAACTC
AATAACGTAAAAGAAAAAATGTAACCTTCTTGTCGATGTAATCTGCCCAAAATCTAGCTT
GAGCTCTCTGGTAAGGATCTGAAGGAAGAATCGGGTTTGTGTCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11