Sequences from confirmation amplicon


Confirmation sequences for line 366H03 and hit At5g03650 are shown.
Line ID 366H03
Locus At5g03650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:936905
Primers used for PCR 19et - 8474, show primer details
   
Sequence Name 57-K110926-0022-366-H03C-19et
GenBank Accession LN509450 [GenBank]
Sequencing primer gene specific primer, 19et: 5'-GATTGGTCAAGAGATTGGAACAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K110926-0022-366-H03C-19et
GTTTATTTTTCTTACATAAGTGAAGTACTCTGCCTTGCGATCGAGCCTATTGAATCCACC
AAAGAGAGGATCGTCCGAGTCCAATACGATCTGGAATGTCGAAATGTAGAAAGATATCGG
ATTCTATCTTCACAACCAATACCAAAGTCTCCAGTGTTTGAAAAGGAAGAGTTAGATGTG
TATTATACCTTATATTTTCCAGGCTTGGAGCAACCAATGCGGTAATCAAAGTAGCTGCTG
GTCCAGTGAAAGTTAAAGACAAAGACGAGATCACCTCTTTCGAATACGATTACTCTATCT
GCTTCGTCTTTTCGTGATATGAATTGGTGCTCCGAAGTCATAAACTGCTCAAATGTAAAA
TAAGAATAAAGCTAATGGGTAACTGAGATCATCTACAAAGGTTTTAACTTACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 41-K110925-0022-366-H03C-8409
GenBank Accession LN504115 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110925-0022-366-H03C-8409
GCCTTTACAATTGAATTATCCTGTAAGTTAAACCTTTGTAGATGATCTCAGTTACCCATT
AGCTTTATTCTTATTTTACATTTGAGCAGTTTATGACTTCGGAGCACCAATTCATATCAC
GAAAAGACGAAGCAGATAGAGTAATCGTATTCGAAAGAGGTGATCTCGTCTTTGTCTTTA
ACTTTCACTGGACCAGCAGCTACTTTGATTACCGCATTGGTTGCTCCAAGCCTGGAAAAT
ATAAGGTATAATACACATCTAACTCTTCCTTTTCAAACACTGGAGACTTTGGTATTGGTT
GTGAAGATAGAATCCGATATCTTTCTACATTTCGACATTCCAGATCGTATTGGACTCGGA
CGATCCTCTCTTTGGTGGATTCAATAGGCTCGATCGCAAGGCAGAGTACTTCACTTATGT
AAGAAAAATAAAACCATTCTAGTCAAAGTTTACATTAATTGTGACTGTTCCAATCTTNGN
ACCAAT

Last Updated on 06.12.2018 16:11