Genotyping and primer details


Genotyping and primer details for 366H03 - At5g03650
Line ID 366H03
Locus At5g03650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:936905 of line 366H03 Chr5:936905
   
Insertion allele amplicon 8474 - 19et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19et: 5'-GATTGGTCAAGAGATTGGAACAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:937368
   
wt amplicon (over InsPos) 12fo - 19et
wt amplicon start Chr5:936481
wt amplicon end Chr5:937368
wt amplicon size 888 bp
Locus specific primer 19et: 5'-GATTGGTCAAGAGATTGGAACAGT-3'
2nd locus specific primer 12fo: 5'-TATAACTATGGGATTAGGCGGTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11